Restriction map for
pF1KB6803
(Linear) (Six-base) MAPSORT of: KIBB6803.seq Check: 1860 from: 1 to: 477
KIBB6803.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 0 128 477
Size: 128 349
AfeI AGC'GCT
Cuts at: 0 74 477
Size: 74 403
AlwNI CAG_nnn'CTG
Cuts at: 0 300 477
Size: 300 177
AvaI C'yCGr_G
Cuts at: 0 472 477
Size: 472 5
BamHI G'GATC_C
Cuts at: 0 393 477
Size: 393 84
BanI G'GyrC_C
Cuts at: 0 61 316 453 477
Size: 61 255 137 24
Fragments arranged by size:
255 137 61 24
BbeI G_GCGC'C
Cuts at: 0 320 477
Size: 320 157
BlpI GC'TnA_GC
Cuts at: 0 412 477
Size: 412 65
Bme1580I G_kGCm'C
Cuts at: 0 458 477
Size: 458 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 459 477
Size: 459 18
BsaHI Gr'CG_yC
Cuts at: 0 125 317 383 477
Size: 125 192 66 94
Fragments arranged by size:
192 125 94 66
BsaWI w'CCGG_w
Cuts at: 0 36 477
Size: 36 441
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 211 340 442 477
Size: 211 129 102 35
Fragments arranged by size:
211 129 102 35
BseYI C'CCAG_C
Cuts at: 0 457 477
Size: 457 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 334 477
Size: 313 21 143
Fragments arranged by size:
313 143 21
Bsp1286I G_dGCh'C
Cuts at: 0 458 477
Size: 458 19
BspEI T'CCGG_A
Cuts at: 0 36 477
Size: 36 441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 477
Size: 356 121
BsrFI r'CCGG_y
Cuts at: 0 121 319 477
Size: 121 198 158
Fragments arranged by size:
198 158 121
BstAPI GCAn_nnn'nTGC
Cuts at: 0 84 477
Size: 84 393
BstBI TT'CG_AA
Cuts at: 0 367 477
Size: 367 110
BstYI r'GATC_y
Cuts at: 0 224 393 477
Size: 224 169 84
Fragments arranged by size:
224 169 84
BtgI C'CryG_G
Cuts at: 0 18 477
Size: 18 459
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 198 302 477
Size: 198 104 175
Fragments arranged by size:
198 175 104
BtsI GCAGTG_nn'
Cuts at: 0 116 351 477
Size: 116 235 126
Fragments arranged by size:
235 126 116
EaeI y'GGCC_r
Cuts at: 0 21 477
Size: 21 456
HaeII r_GCGC'y
Cuts at: 0 76 320 477
Size: 76 244 157
Fragments arranged by size:
244 157 76
HincII GTy'rAC
Cuts at: 0 444 477
Size: 444 33
HpaI GTT'AAC
Cuts at: 0 444 477
Size: 444 33
KasI G'GCGC_C
Cuts at: 0 316 477
Size: 316 161
MspA1I CmG'CkG
Cuts at: 0 20 297 477
Size: 20 277 180
Fragments arranged by size:
277 180 20
NaeI GCC'GGC
Cuts at: 0 123 321 477
Size: 123 198 156
Fragments arranged by size:
198 156 123
NarI GG'CG_CC
Cuts at: 0 317 477
Size: 317 160
NgoMIV G'CCGG_C
Cuts at: 0 121 319 477
Size: 121 198 158
Fragments arranged by size:
198 158 121
PstI C_TGCA'G
Cuts at: 0 224 477
Size: 224 253
SacII CC_GC'GG
Cuts at: 0 21 477
Size: 21 456
SfcI C'TryA_G
Cuts at: 0 220 477
Size: 220 257
SfoI GGC'GCC
Cuts at: 0 318 477
Size: 318 159
SgrAI Cr'CCGG_yG
Cuts at: 0 319 477
Size: 319 158
StyI C'CwwG_G
Cuts at: 0 401 477
Size: 401 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 441 477
Size: 441 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 45 77 477
Size: 45 32 400
Fragments arranged by size:
400 45 32
Enzymes that do cut:
AccI AfeI AlwNI AvaI BamHI BanI BbeI BlpI
Bme1580I BmrI BsaHI BsaWI BseRI BseYI BsgI Bsp1286I
BspEI BspMI BsrFI BstAPI BstBI BstYI BtgI BtgZI
BtsI EaeI HaeII HincII HpaI KasI MspA1I NaeI
NarI NgoMIV PstI SacII SfcI SfoI SgrAI StyI
TaqII TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII KpnI MfeI MluI MmeI MscI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI