Restriction map for pF1KB6804
 (Linear) (Six-base) MAPSORT of: KIBB6804.seq  Check: 2963  from: 1  to: 552



KIBB6804.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     285     552 
   Size:        285     267
 
ApoI r'AATT_y

Cuts at:      0      77     396     552 
   Size:         77     319     156

  Fragments arranged by size: 

                 319     156      77
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     162     552 
   Size:        162     390
 
BsrDI GCAATG_nn'

Cuts at:      0     203     552 
   Size:        203     349
 
Bsu36I CC'TnA_GG

Cuts at:      0     286     552 
   Size:        286     266
 
EaeI y'GGCC_r

Cuts at:      0     222     552 
   Size:        222     330
 
EcoO109I rG'GnC_Cy

Cuts at:      0     282     552 
   Size:        282     270
 
PfoI T'CCnGG_A

Cuts at:      0     164     552 
   Size:        164     388
 
PpuMI rG'GwC_Cy

Cuts at:      0     282     552 
   Size:        282     270
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     284     316     552 
   Size:        284      32     236

  Fragments arranged by size: 

                 284     236      32
 
PstI C_TGCA'G

Cuts at:      0     177     552 
   Size:        177     375
 
SexAI A'CCwGG_T

Cuts at:      0     479     552 
   Size:        479      73
 
SfcI C'TryA_G

Cuts at:      0     117     173     552 
   Size:        117      56     379

  Fragments arranged by size: 

                 379     117      56
 
SpeI A'CTAG_T

Cuts at:      0     237     552 
   Size:        237     315
 
StyI C'CwwG_G

Cuts at:      0      24     225     552 
   Size:         24     201     327

  Fragments arranged by size: 

                 327     201      24
 
TatI w'GTAC_w

Cuts at:      0       8     552 
   Size:          8     544
 
Tth111I GACn'n_nGTC

Cuts at:      0     481     552 
   Size:        481      71
 
XmnI GAAnn'nnTTC

Cuts at:      0     323     552 
   Size:        323     229
 

 Enzymes that do cut:

    AlwNI     ApoI    BciVI    BsrDI   Bsu36I     EaeI EcoO109I     PfoI
    PpuMI     PsrI     PstI    SexAI     SfcI     SpeI     StyI     TatI
  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI     XbaI     XcmI     XhoI
     XmaI     ZraI
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