(Linear) (Six-base) MAPSORT of: KIBB6804.seq Check: 2963 from: 1 to: 552 KIBB6804.orf With 182 enzymes: SgfI * June 26, 2008 10:48 .. AlwNI CAG_nnn'CTG Cuts at: 0 285 552 Size: 285 267 ApoI r'AATT_y Cuts at: 0 77 396 552 Size: 77 319 156 Fragments arranged by size: 319 156 77 BciVI GTATCCnnnnn_n' Cuts at: 0 162 552 Size: 162 390 BsrDI GCAATG_nn' Cuts at: 0 203 552 Size: 203 349 Bsu36I CC'TnA_GG Cuts at: 0 286 552 Size: 286 266 EaeI y'GGCC_r Cuts at: 0 222 552 Size: 222 330 EcoO109I rG'GnC_Cy Cuts at: 0 282 552 Size: 282 270 PfoI T'CCnGG_A Cuts at: 0 164 552 Size: 164 388 PpuMI rG'GwC_Cy Cuts at: 0 282 552 Size: 282 270 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 284 316 552 Size: 284 32 236 Fragments arranged by size: 284 236 32 PstI C_TGCA'G Cuts at: 0 177 552 Size: 177 375 SexAI A'CCwGG_T Cuts at: 0 479 552 Size: 479 73 SfcI C'TryA_G Cuts at: 0 117 173 552 Size: 117 56 379 Fragments arranged by size: 379 117 56 SpeI A'CTAG_T Cuts at: 0 237 552 Size: 237 315 StyI C'CwwG_G Cuts at: 0 24 225 552 Size: 24 201 327 Fragments arranged by size: 327 201 24 TatI w'GTAC_w Cuts at: 0 8 552 Size: 8 544 Tth111I GACn'n_nGTC Cuts at: 0 481 552 Size: 481 71 XmnI GAAnn'nnTTC Cuts at: 0 323 552 Size: 323 229 Enzymes that do cut: AlwNI ApoI BciVI BsrDI Bsu36I EaeI EcoO109I PfoI PpuMI PsrI PstI SexAI SfcI SpeI StyI TatI Tth111I XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI SwaI TaqII TstI XbaI XcmI XhoI XmaI ZraI