(Linear) (Six-base) MAPSORT of: KIBB6805.seq Check: 8496 from: 1 to: 477 KIBB6805.orf With 182 enzymes: SgfI * October 7, 2009 14:23 .. AleI CACnn'nnGTG Cuts at: 0 400 477 Size: 400 77 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 120 152 477 Size: 120 32 325 Fragments arranged by size: 325 120 32 BbsI GAAGACnn'nnnn_ Cuts at: 0 353 477 Size: 353 124 Bme1580I G_kGCm'C Cuts at: 0 114 477 Size: 114 363 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 231 477 Size: 231 246 BsaI GGTCTCn'nnnn_ Cuts at: 0 355 421 477 Size: 355 66 56 Fragments arranged by size: 355 66 56 Bsp1286I G_dGCh'C Cuts at: 0 114 477 Size: 114 363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 373 477 Size: 373 104 BstXI CCAn_nnnn'nTGG Cuts at: 0 438 477 Size: 438 39 BstYI r'GATC_y Cuts at: 0 147 477 Size: 147 330 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 211 243 477 Size: 211 32 234 Fragments arranged by size: 234 211 32 HincII GTy'rAC Cuts at: 0 93 477 Size: 93 384 MslI CAynn'nnrTG Cuts at: 0 400 477 Size: 400 77 MspA1I CmG'CkG Cuts at: 0 400 477 Size: 400 77 PfoI T'CCnGG_A Cuts at: 0 124 477 Size: 124 353 PvuII CAG'CTG Cuts at: 0 400 477 Size: 400 77 SfcI C'TryA_G Cuts at: 0 287 356 477 Size: 287 69 121 Fragments arranged by size: 287 121 69 Enzymes that do cut: AleI AloI BbsI Bme1580I BplI BsaI Bsp1286I BspMI BstXI BstYI Hin4I HincII MslI MspA1I PfoI PvuII SfcI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI