(Linear) (Six-base) MAPSORT of: KIBB6809.seq  Check: 5028  from: 1  to: 477



KIBB6809.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:      0     317     477 
   Size:        317     160
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     477 
   Size:         43     434
 
AflIII A'CryG_T

Cuts at:      0     323     477 
   Size:        323     154
 
ApaLI G'TGCA_C

Cuts at:      0     208     477 
   Size:        208     269
 
AvaI C'yCGr_G

Cuts at:      0     467     477 
   Size:        467      10
 
AvrII C'CTAG_G

Cuts at:      0      36     477 
   Size:         36     441
 
BanI G'GyrC_C

Cuts at:      0     420     477 
   Size:        420      57
 
BanII G_rGCy'C

Cuts at:      0     350     477 
   Size:        350     127
 
BbeI G_GCGC'C

Cuts at:      0     424     477 
   Size:        424      53
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     368     477 
   Size:        368     109
 
BbvCI CC'TCA_GC

Cuts at:      0     280     477 
   Size:        280     197
 
BglI GCCn_nnn'nGGC

Cuts at:      0     260     477 
   Size:        260     217
 
BlpI GC'TnA_GC

Cuts at:      0     137     477 
   Size:        137     340
 
Bme1580I G_kGCm'C

Cuts at:      0     212     477 
   Size:        212     265
 
Bpu10I CC'TnA_GC

Cuts at:      0     280     477 
   Size:        280     197
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     477 
   Size:        277     200
 
BsaHI Gr'CG_yC

Cuts at:      0     155     421     477 
   Size:        155     266      56

  Fragments arranged by size: 

                 266     155      56
 
BsiHKAI G_wGCw'C

Cuts at:      0     212     350     477 
   Size:        212     138     127

  Fragments arranged by size: 

                 212     138     127
 
Bsp1286I G_dGCh'C

Cuts at:      0     212     350     477 
   Size:        212     138     127

  Fragments arranged by size: 

                 212     138     127
 
BsrBI CCG'CTC

Cuts at:      0     300     477 
   Size:        300     177
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     198     477 
   Size:        198     279
 
BstYI r'GATC_y

Cuts at:      0     429     477 
   Size:        429      48
 
BtgI C'CryG_G

Cuts at:      0     229     477 
   Size:        229     248
 
EaeI y'GGCC_r

Cuts at:      0     185     272     477 
   Size:        185      87     205

  Fragments arranged by size: 

                 205     185      87
 
EcoICRI GAG'CTC

Cuts at:      0     348     477 
   Size:        348     129
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     477 
   Size:         43     434
 
HaeII r_GCGC'y

Cuts at:      0     424     477 
   Size:        424      53
 
KasI G'GCGC_C

Cuts at:      0     420     477 
   Size:        420      57
 
MscI TGG'CCA

Cuts at:      0     187     274     477 
   Size:        187      87     203

  Fragments arranged by size: 

                 203     187      87
 
MslI CAynn'nnrTG

Cuts at:      0     163     477 
   Size:        163     314
 
MspA1I CmG'CkG

Cuts at:      0     141     231     477 
   Size:        141      90     246

  Fragments arranged by size: 

                 246     141      90
 
NarI GG'CG_CC

Cuts at:      0     421     477 
   Size:        421      56
 
NspI r_CATG'y

Cuts at:      0     168     327     477 
   Size:        168     159     150

  Fragments arranged by size: 

                 168     159     150
 
PciI A'CATG_T

Cuts at:      0     323     477 
   Size:        323     154
 
PfoI T'CCnGG_A

Cuts at:      0     197     477 
   Size:        197     280
 
SacI G_AGCT'C

Cuts at:      0     350     477 
   Size:        350     127
 
SacII CC_GC'GG

Cuts at:      0     232     477 
   Size:        232     245
 
SfoI GGC'GCC

Cuts at:      0     422     477 
   Size:        422      55
 
SmlI C'TyrA_G

Cuts at:      0     292     477 
   Size:        292     185
 
StyI C'CwwG_G

Cuts at:      0      36     116     369     477 
   Size:         36      80     253     108

  Fragments arranged by size: 

                 253     108      80      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     163     477 
   Size:        163     314
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     114     195     477 
   Size:        114      81     282

  Fragments arranged by size: 

                 282     114      81
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    ApaLI     AvaI    AvrII     BanI    BanII
     BbeI     BbsI    BbvCI     BglI     BlpI Bme1580I   Bpu10I    BpuEI
    BsaHI  BsiHKAI Bsp1286I    BsrBI    BstXI    BstYI     BtgI     EaeI
  EcoICRI  Eco57MI    HaeII     KasI     MscI     MslI   MspA1I     NarI
     NspI     PciI     PfoI     SacI    SacII     SfoI     SmlI     StyI
    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI