Restriction map for
pF1KB6812
(Linear) (Six-base) MAPSORT of: KIBB6812.seq Check: 9497 from: 1 to: 555
KIBB6812.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 555
Size: 110 445
AflIII A'CryG_T
Cuts at: 0 408 555
Size: 408 147
AlwNI CAG_nnn'CTG
Cuts at: 0 216 306 555
Size: 216 90 249
Fragments arranged by size:
249 216 90
ApaI G_GGCC'C
Cuts at: 0 76 124 193 555
Size: 76 48 69 362
Fragments arranged by size:
362 76 69 48
AvaI C'yCGr_G
Cuts at: 0 476 555
Size: 476 79
BanI G'GyrC_C
Cuts at: 0 25 252 555
Size: 25 227 303
Fragments arranged by size:
303 227 25
BanII G_rGCy'C
Cuts at: 0 76 124 193 477 555
Size: 76 48 69 284 78
Fragments arranged by size:
284 78 76 69 48
BbeI G_GCGC'C
Cuts at: 0 29 555
Size: 29 526
BglI GCCn_nnn'nGGC
Cuts at: 0 12 40 197 535 555
Size: 12 28 157 338 20
Fragments arranged by size:
338 157 28 20 12
Bme1580I G_kGCm'C
Cuts at: 0 76 124 193 555
Size: 76 48 69 362
Fragments arranged by size:
362 76 69 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 118 146 489 555
Size: 118 28 343 66
Fragments arranged by size:
343 118 66 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 555
Size: 458 97
Bpu10I CC'TnA_GC
Cuts at: 0 77 555
Size: 77 478
BsaHI Gr'CG_yC
Cuts at: 0 26 555
Size: 26 529
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 260 290 555
Size: 260 30 265
Fragments arranged by size:
265 260 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 74 169 253 370 555
Size: 74 95 84 117 185
Fragments arranged by size:
185 117 95 84 74
BseYI C'CCAG_C
Cuts at: 0 192 555
Size: 192 363
Bsp1286I G_dGCh'C
Cuts at: 0 76 124 193 477 555
Size: 76 48 69 284 78
Fragments arranged by size:
284 78 76 69 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 544 555
Size: 544 11
BstYI r'GATC_y
Cuts at: 0 225 555
Size: 225 330
Bsu36I CC'TnA_GG
Cuts at: 0 161 555
Size: 161 394
BtgI C'CryG_G
Cuts at: 0 68 395 555
Size: 68 327 160
Fragments arranged by size:
327 160 68
BtsI GCAGTG_nn'
Cuts at: 0 156 555
Size: 156 399
EarI CTCTTCn'nnn_
Cuts at: 0 161 555
Size: 161 394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 458 555
Size: 110 348 97
Fragments arranged by size:
348 110 97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 128 455 555
Size: 128 327 100
Fragments arranged by size:
327 128 100
EcoO109I rG'GnC_Cy
Cuts at: 0 4 73 120 121 303 555
Size: 4 69 47 1 182 252
Fragments arranged by size:
252 182 69 47 4 1
EcoRV GAT'ATC
Cuts at: 0 485 555
Size: 485 70
HaeII r_GCGC'y
Cuts at: 0 29 555
Size: 29 526
KasI G'GCGC_C
Cuts at: 0 25 555
Size: 25 530
MspA1I CmG'CkG
Cuts at: 0 196 555
Size: 196 359
NarI GG'CG_CC
Cuts at: 0 26 555
Size: 26 529
NcoI C'CATG_G
Cuts at: 0 68 395 555
Size: 68 327 160
Fragments arranged by size:
327 160 68
NspI r_CATG'y
Cuts at: 0 412 555
Size: 412 143
PasI CC'CwG_GG
Cuts at: 0 306 555
Size: 306 249
PciI A'CATG_T
Cuts at: 0 408 555
Size: 408 147
PflMI CCAn_nnn'nTGG
Cuts at: 0 306 555
Size: 306 249
PfoI T'CCnGG_A
Cuts at: 0 287 555
Size: 287 268
PspOMI G'GGCC_C
Cuts at: 0 72 120 189 555
Size: 72 48 69 366
Fragments arranged by size:
366 72 69 48
PvuII CAG'CTG
Cuts at: 0 196 555
Size: 196 359
SapI GCTCTTCn'nnn_
Cuts at: 0 161 555
Size: 161 394
SfcI C'TryA_G
Cuts at: 0 365 555
Size: 365 190
SfoI GGC'GCC
Cuts at: 0 27 555
Size: 27 528
StyI C'CwwG_G
Cuts at: 0 68 395 555
Size: 68 327 160
Fragments arranged by size:
327 160 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 481 513 555
Size: 481 32 42
Fragments arranged by size:
481 42 32
Enzymes that do cut:
AcuI AflIII AlwNI ApaI AvaI BanI BanII BbeI
BglI Bme1580I BmrI BpmI Bpu10I BsaHI BsaXI BseRI
BseYI Bsp1286I BstAPI BstYI Bsu36I BtgI BtsI EarI
Eco57MI EcoNI EcoO109I EcoRV HaeII KasI MspA1I NarI
NcoI NspI PasI PciI PflMI PfoI PspOMI PvuII
SapI SfcI SfoI StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoRI FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI