(Linear) (Six-base) MAPSORT of: KIBB6814.seq  Check: 2380  from: 1  to: 555



KIBB6814.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

ApoI r'AATT_y

Cuts at:      0     320     505     555 
   Size:        320     185      50

  Fragments arranged by size: 

                 320     185      50
 
BanII G_rGCy'C

Cuts at:      0     496     555 
   Size:        496      59
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     229     555 
   Size:        229     326
 
BglII A'GATC_T

Cuts at:      0     278     555 
   Size:        278     277
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     555 
   Size:        352     203
 
BsaBI GATnn'nnATC

Cuts at:      0     143     555 
   Size:        143     412
 
BseYI C'CCAG_C

Cuts at:      0     176     555 
   Size:        176     379
 
BsiHKAI G_wGCw'C

Cuts at:      0     496     555 
   Size:        496      59
 
Bsp1286I G_dGCh'C

Cuts at:      0     496     555 
   Size:        496      59
 
BspHI T'CATG_A

Cuts at:      0     404     533     555 
   Size:        404     129      22

  Fragments arranged by size: 

                 404     129      22
 
BsrGI T'GTAC_A

Cuts at:      0     517     555 
   Size:        517      38
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      45     353     555 
   Size:         45     308     202

  Fragments arranged by size: 

                 308     202      45
 
BstYI r'GATC_y

Cuts at:      0     278     555 
   Size:        278     277
 
EarI CTCTTCn'nnn_

Cuts at:      0     438     482     555 
   Size:        438      44      73

  Fragments arranged by size: 

                 438      73      44
 
EcoICRI GAG'CTC

Cuts at:      0     494     555 
   Size:        494      61
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     235     267     555 
   Size:        235      32     288

  Fragments arranged by size: 

                 288     235      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     555 
   Size:         26     529
 
MslI CAynn'nnrTG

Cuts at:      0     437     454     555 
   Size:        437      17     101

  Fragments arranged by size: 

                 437     101      17
 
NdeI CA'TA_TG

Cuts at:      0     455     555 
   Size:        455     100
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     118     555 
   Size:        118     437
 
SacI G_AGCT'C

Cuts at:      0     496     555 
   Size:        496      59
 
SfcI C'TryA_G

Cuts at:      0     524     555 
   Size:        524      31
 
SmlI C'TyrA_G

Cuts at:      0     331     555 
   Size:        331     224
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     466     555 
   Size:        466      89
 
TatI w'GTAC_w

Cuts at:      0     517     555 
   Size:        517      38
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     107     139     555 
   Size:        107      32     416

  Fragments arranged by size: 

                 416     107      32
 
XbaI T'CTAG_A

Cuts at:      0     147     555 
   Size:        147     408
 

 Enzymes that do cut:

     ApoI    BanII     BbsI    BglII    BpuEI    BsaBI    BseYI  BsiHKAI
 Bsp1286I    BspHI    BsrGI    BstXI    BstYI     EarI  EcoICRI    Hin4I
     MmeI     MslI     NdeI    PflMI     SacI     SfcI     SmlI    TaqII
     TatI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI