(Linear) (Six-base) MAPSORT of: KIBB6816.seq Check: 8766 from: 1 to: 555 KIBB6816.orf With 182 enzymes: SgfI * April 24, 2009 13:06 .. AccI GT'mk_AC Cuts at: 0 211 312 555 Size: 211 101 243 Fragments arranged by size: 243 211 101 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 319 555 Size: 64 255 236 Fragments arranged by size: 255 236 64 AlwNI CAG_nnn'CTG Cuts at: 0 238 555 Size: 238 317 ApoI r'AATT_y Cuts at: 0 365 555 Size: 365 190 BciVI GTATCCnnnnn_n' Cuts at: 0 189 555 Size: 189 366 BspHI T'CATG_A Cuts at: 0 516 555 Size: 516 39 BtsI GCAGTG_nn' Cuts at: 0 105 509 555 Size: 105 404 46 Fragments arranged by size: 404 105 46 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 319 555 Size: 64 255 236 Fragments arranged by size: 255 236 64 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 265 297 555 Size: 265 32 258 Fragments arranged by size: 265 258 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 518 555 Size: 518 37 MspA1I CmG'CkG Cuts at: 0 339 555 Size: 339 216 PvuII CAG'CTG Cuts at: 0 339 555 Size: 339 216 SfcI C'TryA_G Cuts at: 0 547 555 Size: 547 8 StuI AGG'CCT Cuts at: 0 48 555 Size: 48 507 XmnI GAAnn'nnTTC Cuts at: 0 77 555 Size: 77 478 Enzymes that do cut: AccI AcuI AlwNI ApoI BciVI BspHI BtsI Eco57MI Hin4I MmeI MspA1I PvuII SfcI StuI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI