Restriction map for pF1KB6816
 (Linear) (Six-base) MAPSORT of: KIBB6816.seq  Check: 8766  from: 1  to: 555



KIBB6816.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:      0     211     312     555 
   Size:        211     101     243

  Fragments arranged by size: 

                 243     211     101
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     319     555 
   Size:         64     255     236

  Fragments arranged by size: 

                 255     236      64
 
AlwNI CAG_nnn'CTG

Cuts at:      0     238     555 
   Size:        238     317
 
ApoI r'AATT_y

Cuts at:      0     365     555 
   Size:        365     190
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     189     555 
   Size:        189     366
 
BspHI T'CATG_A

Cuts at:      0     516     555 
   Size:        516      39
 
BtsI GCAGTG_nn'

Cuts at:      0     105     509     555 
   Size:        105     404      46

  Fragments arranged by size: 

                 404     105      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     319     555 
   Size:         64     255     236

  Fragments arranged by size: 

                 255     236      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     265     297     555 
   Size:        265      32     258

  Fragments arranged by size: 

                 265     258      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     518     555 
   Size:        518      37
 
MspA1I CmG'CkG

Cuts at:      0     339     555 
   Size:        339     216
 
PvuII CAG'CTG

Cuts at:      0     339     555 
   Size:        339     216
 
SfcI C'TryA_G

Cuts at:      0     547     555 
   Size:        547       8
 
StuI AGG'CCT

Cuts at:      0      48     555 
   Size:         48     507
 
XmnI GAAnn'nnTTC

Cuts at:      0      77     555 
   Size:         77     478
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI    BciVI    BspHI     BtsI  Eco57MI
    Hin4I     MmeI   MspA1I    PvuII     SfcI     StuI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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