(Linear) (Six-base) MAPSORT of: KIBB6818.seq  Check: 8537  from: 1  to: 558



KIBB6818.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     181     558 
   Size:        181     377
 
ApoI r'AATT_y

Cuts at:      0     475     558 
   Size:        475      83
 
AvaI C'yCGr_G

Cuts at:      0      31     558 
   Size:         31     527
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     208     558 
   Size:        208     350
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     328     558 
   Size:        328     230
 
BglI GCCn_nnn'nGGC

Cuts at:      0     238     558 
   Size:        238     320
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     558 
   Size:        112     446
 
BseYI C'CCAG_C

Cuts at:      0      74     558 
   Size:         74     484
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     412     558 
   Size:        172     240     146

  Fragments arranged by size: 

                 240     172     146
 
BspHI T'CATG_A

Cuts at:      0     513     558 
   Size:        513      45
 
BssSI C'ACGA_G

Cuts at:      0     149     558 
   Size:        149     409
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     226     558 
   Size:        226     332
 
BtgI C'CryG_G

Cuts at:      0      60     558 
   Size:         60     498
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     219     558 
   Size:        219     339
 
BtsI GCAGTG_nn'

Cuts at:      0      26     361     558 
   Size:         26     335     197

  Fragments arranged by size: 

                 335     197      26
 
EaeI y'GGCC_r

Cuts at:      0     216     239     446     489     558 
   Size:        216      23     207      43      69

  Fragments arranged by size: 

                 216     207      69      43      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     558 
   Size:        112     446
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     143     175     558 
   Size:        143      32     383

  Fragments arranged by size: 

                 383     143      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     437     558 
   Size:        437     121
 
MscI TGG'CCA

Cuts at:      0     218     491     558 
   Size:        218     273      67

  Fragments arranged by size: 

                 273     218      67
 
MslI CAynn'nnrTG

Cuts at:      0     194     558 
   Size:        194     364
 
NspI r_CATG'y

Cuts at:      0     132     558 
   Size:        132     426
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     117     558 
   Size:        117     441
 
SexAI A'CCwGG_T

Cuts at:      0     102     558 
   Size:        102     456
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     238     558 
   Size:        238     320
 
SmaI CCC'GGG

Cuts at:      0      33     558 
   Size:         33     525
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      66      73     558 
   Size:         66       7     485

  Fragments arranged by size: 

                 485      66       7
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      67     558 
   Size:         67     491
 
XmaI C'CCGG_G

Cuts at:      0      31     558 
   Size:         31     527
 

 Enzymes that do cut:

     AlfI     ApoI     AvaI     BbsI    BciVI     BglI     BpmI    BseYI
     BsgI    BspHI    BssSI    BstXI     BtgI    BtgZI     BtsI     EaeI
  Eco57MI    Hin4I     MmeI     MscI     MslI     NspI    PflMI    SexAI
     SfiI     SmaI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI