(Linear) (Six-base) MAPSORT of: KIBB6819.seq  Check: 514  from: 1  to: 486



KIBB6819.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     486 
   Size:        101     385
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     224     282     486 
   Size:        224      58     204

  Fragments arranged by size: 

                 224     204      58
 
AlwNI CAG_nnn'CTG

Cuts at:      0     391     486 
   Size:        391      95
 
ApoI r'AATT_y

Cuts at:      0       5     436     486 
   Size:          5     431      50

  Fragments arranged by size: 

                 431      50       5
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      90     359     486 
   Size:         90     269     127

  Fragments arranged by size: 

                 269     127      90
 
BclI T'GATC_A

Cuts at:      0     341     486 
   Size:        341     145
 
BfrBI ATG'CAT

Cuts at:      0     201     486 
   Size:        201     285
 
BglII A'GATC_T

Cuts at:      0     183     486 
   Size:        183     303
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     156     236     486 
   Size:        156      80     250

  Fragments arranged by size: 

                 250     156      80
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     486 
   Size:        436      50
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     486 
   Size:        319     167
 
BsaWI w'CCGG_w

Cuts at:      0     304     486 
   Size:        304     182
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     486 
   Size:        140     346
 
BsmI GAATG_Cn'

Cuts at:      0      89     486 
   Size:         89     397
 
BspEI T'CCGG_A

Cuts at:      0     304     486 
   Size:        304     182
 
BspHI T'CATG_A

Cuts at:      0     321     486 
   Size:        321     165
 
BsrDI GCAATG_nn'

Cuts at:      0     266     486 
   Size:        266     220
 
BsrGI T'GTAC_A

Cuts at:      0     453     486 
   Size:        453      33
 
BstBI TT'CG_AA

Cuts at:      0      10     486 
   Size:         10     476
 
BstYI r'GATC_y

Cuts at:      0     183     486 
   Size:        183     303
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     436     486 
   Size:        101     335      50

  Fragments arranged by size: 

                 335     101      50
 
MslI CAynn'nnrTG

Cuts at:      0     206     486 
   Size:        206     280
 
NsiI A_TGCA'T

Cuts at:      0     203     486 
   Size:        203     283
 
SmlI C'TyrA_G

Cuts at:      0     298     486 
   Size:        298     188
 
SspI AAT'ATT

Cuts at:      0     429     486 
   Size:        429      57
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     138     486 
   Size:        138     348
 
TatI w'GTAC_w

Cuts at:      0     453     486 
   Size:        453      33
 
XmnI GAAnn'nnTTC

Cuts at:      0      30     486 
   Size:         30     456
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApoI     BbsI     BclI    BfrBI    BglII
     BmrI     BpmI    BpuEI    BsaWI     BsgI     BsmI    BspEI    BspHI
    BsrDI    BsrGI    BstBI    BstYI  Eco57MI     MslI     NsiI     SmlI
     SspI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BglI     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI