(Linear) (Six-base) MAPSORT of: KIBB6821.seq  Check: 7664  from: 1  to: 489



KIBB6821.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

ApaI G_GGCC'C

Cuts at:      0      35     489 
   Size:         35     454
 
ApoI r'AATT_y

Cuts at:      0     208     316     489 
   Size:        208     108     173

  Fragments arranged by size: 

                 208     173     108
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     439     472     489 
   Size:        439      33      17

  Fragments arranged by size: 

                 439      33      17
 
BanII G_rGCy'C

Cuts at:      0      35     408     489 
   Size:         35     373      81

  Fragments arranged by size: 

                 373      81      35
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      42      76     489 
   Size:         42      34     413

  Fragments arranged by size: 

                 413      42      34
 
Bme1580I G_kGCm'C

Cuts at:      0      35     489 
   Size:         35     454
 
BsaWI w'CCGG_w

Cuts at:      0     164     489 
   Size:        164     325
 
BsmI GAATG_Cn'

Cuts at:      0     160     489 
   Size:        160     329
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     408     489 
   Size:         35     373      81

  Fragments arranged by size: 

                 373      81      35
 
BspEI T'CCGG_A

Cuts at:      0     164     489 
   Size:        164     325
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      74     489 
   Size:         74     415
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     289     489 
   Size:        289     200
 
DraI TTT'AAA

Cuts at:      0     120     489 
   Size:        120     369
 
EarI CTCTTCn'nnn_

Cuts at:      0     274     431     489 
   Size:        274     157      58

  Fragments arranged by size: 

                 274     157      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     413     489 
   Size:        413      76
 
EcoO109I rG'GnC_Cy

Cuts at:      0      31     489 
   Size:         31     458
 
EcoRV GAT'ATC

Cuts at:      0     369     489 
   Size:        369     120
 
HaeII r_GCGC'y

Cuts at:      0     309     489 
   Size:        309     180
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     489 
   Size:        413      76
 
MspA1I CmG'CkG

Cuts at:      0     175     489 
   Size:        175     314
 
PfoI T'CCnGG_A

Cuts at:      0      42     489 
   Size:         42     447
 
PsiI TTA'TAA

Cuts at:      0     322     489 
   Size:        322     167
 
PspOMI G'GGCC_C

Cuts at:      0      31     489 
   Size:         31     458
 
PvuII CAG'CTG

Cuts at:      0     175     489 
   Size:        175     314
 
SfcI C'TryA_G

Cuts at:      0     352     489 
   Size:        352     137
 

 Enzymes that do cut:

     ApaI     ApoI     BaeI    BanII     BcgI Bme1580I    BsaWI     BsmI
 Bsp1286I    BspEI   BstAPI    BtgZI     DraI     EarI    EcoNI EcoO109I
    EcoRV    HaeII     MmeI   MspA1I     PfoI     PsiI   PspOMI    PvuII
     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI  Eco57MI    EcoRI     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI