Restriction map for
pF1KB6822
(Linear) (Six-base) MAPSORT of: KIBB6822.seq Check: 3873 from: 1 to: 558
KIBB6822.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 111 297 558
Size: 111 186 261
Fragments arranged by size:
261 186 111
AfeI AGC'GCT
Cuts at: 0 55 382 558
Size: 55 327 176
Fragments arranged by size:
327 176 55
AleI CACnn'nnGTG
Cuts at: 0 538 558
Size: 538 20
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 329 558
Size: 329 229
AvaI C'yCGr_G
Cuts at: 0 35 558
Size: 35 523
BamHI G'GATC_C
Cuts at: 0 204 558
Size: 204 354
BanI G'GyrC_C
Cuts at: 0 321 558
Size: 321 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 242 558
Size: 208 34 316
Fragments arranged by size:
316 208 34
BclI T'GATC_A
Cuts at: 0 191 558
Size: 191 367
BglII A'GATC_T
Cuts at: 0 186 558
Size: 186 372
Bme1580I G_kGCm'C
Cuts at: 0 326 558
Size: 326 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 322 472 558
Size: 70 252 150 86
Fragments arranged by size:
252 150 86 70
Bpu10I CC'TnA_GC
Cuts at: 0 359 558
Size: 359 199
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 448 558
Size: 283 165 110
Fragments arranged by size:
283 165 110
BseYI C'CCAG_C
Cuts at: 0 56 558
Size: 56 502
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 517 558
Size: 139 378 41
Fragments arranged by size:
378 139 41
BsiEI CG_ry'CG
Cuts at: 0 222 558
Size: 222 336
BsiHKAI G_wGCw'C
Cuts at: 0 342 558
Size: 342 216
BsmI GAATG_Cn'
Cuts at: 0 318 558
Size: 318 240
Bsp1286I G_dGCh'C
Cuts at: 0 326 342 558
Size: 326 16 216
Fragments arranged by size:
326 216 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 111 297 558
Size: 111 186 261
Fragments arranged by size:
261 186 111
BsrDI GCAATG_nn'
Cuts at: 0 224 558
Size: 224 334
BssHII G'CGCG_C
Cuts at: 0 79 558
Size: 79 479
BstAPI GCAn_nnn'nTGC
Cuts at: 0 495 558
Size: 495 63
BstYI r'GATC_y
Cuts at: 0 186 204 392 558
Size: 186 18 188 166
Fragments arranged by size:
188 186 166 18
BtsI GCAGTG_nn'
Cuts at: 0 505 558
Size: 505 53
DrdI GACnn_nn'nnGTC
Cuts at: 0 109 558
Size: 109 449
EarI CTCTTCn'nnn_
Cuts at: 0 38 558
Size: 38 520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 322 472 558
Size: 70 252 150 86
Fragments arranged by size:
252 150 86 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 112 265 286 304 558
Size: 112 153 21 18 254
Fragments arranged by size:
254 153 112 21 18
HaeII r_GCGC'y
Cuts at: 0 57 384 558
Size: 57 327 174
Fragments arranged by size:
327 174 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 171 203 558
Size: 171 32 355
Fragments arranged by size:
355 171 32
MslI CAynn'nnrTG
Cuts at: 0 538 558
Size: 538 20
MspA1I CmG'CkG
Cuts at: 0 24 558
Size: 24 534
NspI r_CATG'y
Cuts at: 0 367 558
Size: 367 191
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 558
Size: 420 138
PfoI T'CCnGG_A
Cuts at: 0 299 449 558
Size: 299 150 109
Fragments arranged by size:
299 150 109
PstI C_TGCA'G
Cuts at: 0 380 558
Size: 380 178
SapI GCTCTTCn'nnn_
Cuts at: 0 38 558
Size: 38 520
SfcI C'TryA_G
Cuts at: 0 28 376 558
Size: 28 348 182
Fragments arranged by size:
348 182 28
SphI G_CATG'C
Cuts at: 0 367 558
Size: 367 191
StyI C'CwwG_G
Cuts at: 0 317 440 558
Size: 317 123 118
Fragments arranged by size:
317 123 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 48 558
Size: 48 510
TatI w'GTAC_w
Cuts at: 0 212 558
Size: 212 346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 447 558
Size: 447 111
Enzymes that do cut:
AarI AfeI AleI AlfI AvaI BamHI BanI BcgI
BclI BglII Bme1580I BpmI Bpu10I BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssHII BstAPI
BstYI BtsI DrdI EarI Eco57MI EcoNI HaeII Hin4I
MslI MspA1I NspI PflMI PfoI PstI SapI SfcI
SphI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanII BbeI BbsI BbvCI BciVI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII EaeI EagI EciI EcoICRI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI