(Linear) (Six-base) MAPSORT of: KIBB6823.seq  Check: 4560  from: 1  to: 489



KIBB6823.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     140     489 
   Size:        140     349
 
AlwNI CAG_nnn'CTG

Cuts at:      0     374     489 
   Size:        374     115
 
BamHI G'GATC_C

Cuts at:      0      47     489 
   Size:         47     442
 
BanII G_rGCy'C

Cuts at:      0     486     489 
   Size:        486       3
 
BmgBI CAC'GTC

Cuts at:      0     331     401     489 
   Size:        331      70      88

  Fragments arranged by size: 

                 331      88      70
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      59     303     489 
   Size:         59     244     186

  Fragments arranged by size: 

                 244     186      59
 
BsaAI yAC'GTr

Cuts at:      0     422     489 
   Size:        422      67
 
BsaBI GATnn'nnATC

Cuts at:      0     273     489 
   Size:        273     216
 
BseYI C'CCAG_C

Cuts at:      0     374     489 
   Size:        374     115
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     410     489 
   Size:        410      79
 
BsiHKAI G_wGCw'C

Cuts at:      0      72     489 
   Size:         72     417
 
Bsp1286I G_dGCh'C

Cuts at:      0      72     486     489 
   Size:         72     414       3

  Fragments arranged by size: 

                 414      72       3
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     140     489 
   Size:        140     349
 
BsrDI GCAATG_nn'

Cuts at:      0     312     489 
   Size:        312     177
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      85     489 
   Size:         85     404
 
BstYI r'GATC_y

Cuts at:      0      47     444     489 
   Size:         47     397      45

  Fragments arranged by size: 

                 397      47      45
 
BtsI GCAGTG_nn'

Cuts at:      0     193     489 
   Size:        193     296
 
DraIII CAC_nnn'GTG

Cuts at:      0     477     489 
   Size:        477      12
 
EaeI y'GGCC_r

Cuts at:      0       2     489 
   Size:          2     487
 
MscI TGG'CCA

Cuts at:      0       4     489 
   Size:          4     485
 
MspA1I CmG'CkG

Cuts at:      0     308     489 
   Size:        308     181
 
PfoI T'CCnGG_A

Cuts at:      0     456     489 
   Size:        456      33
 
PmlI CAC'GTG

Cuts at:      0     422     489 
   Size:        422      67
 
PstI C_TGCA'G

Cuts at:      0      81     257     489 
   Size:         81     176     232

  Fragments arranged by size: 

                 232     176      81
 
PvuII CAG'CTG

Cuts at:      0     308     489 
   Size:        308     181
 
SfcI C'TryA_G

Cuts at:      0      77     253     489 
   Size:         77     176     236

  Fragments arranged by size: 

                 236     176      77
 
StyI C'CwwG_G

Cuts at:      0      11     489 
   Size:         11     478
 

 Enzymes that do cut:

     AarI    AlwNI    BamHI    BanII    BmgBI     BmrI    BsaAI    BsaBI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI   BstAPI    BstYI
     BtsI   DraIII     EaeI     MscI   MspA1I     PfoI     PmlI     PstI
    PvuII     SfcI     StyI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI