(Linear) (Six-base) MAPSORT of: KIBB6828.seq  Check: 3295  from: 1  to: 564



KIBB6828.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15     564 
   Size:         15     549
 
BanI G'GyrC_C

Cuts at:      0     483     564 
   Size:        483      81
 
BbvCI CC'TCA_GC

Cuts at:      0     420     564 
   Size:        420     144
 
Bme1580I G_kGCm'C

Cuts at:      0     488     564 
   Size:        488      76
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      36     564 
   Size:         36     528
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     564 
   Size:         58     506
 
Bpu10I CC'TnA_GC

Cuts at:      0     420     564 
   Size:        420     144
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      12     564 
   Size:         12     552
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      34     564 
   Size:         34     530
 
Bsp1286I G_dGCh'C

Cuts at:      0     488     564 
   Size:        488      76
 
BsrBI CCG'CTC

Cuts at:      0     198     564 
   Size:        198     366
 
BsrDI GCAATG_nn'

Cuts at:      0     407     564 
   Size:        407     157
 
BssSI C'ACGA_G

Cuts at:      0      24     564 
   Size:         24     540
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     373     564 
   Size:        373     191
 
BtgI C'CryG_G

Cuts at:      0     290     564 
   Size:        290     274
 
BtsI GCAGTG_nn'

Cuts at:      0     365     564 
   Size:        365     199
 
DraI TTT'AAA

Cuts at:      0     473     564 
   Size:        473      91
 
EaeI y'GGCC_r

Cuts at:      0     340     564 
   Size:        340     224
 
EarI CTCTTCn'nnn_

Cuts at:      0     113     506     564 
   Size:        113     393      58

  Fragments arranged by size: 

                 393     113      58
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     310     564 
   Size:        310     254
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15      58     564 
   Size:         15      43     506

  Fragments arranged by size: 

                 506      43      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     160     192     564 
   Size:        160      32     372

  Fragments arranged by size: 

                 372     160      32
 
MscI TGG'CCA

Cuts at:      0     342     564 
   Size:        342     222
 
MslI CAynn'nnrTG

Cuts at:      0     358     412     564 
   Size:        358      54     152

  Fragments arranged by size: 

                 358     152      54
 
NcoI C'CATG_G

Cuts at:      0     290     564 
   Size:        290     274
 
NdeI CA'TA_TG

Cuts at:      0     490     564 
   Size:        490      74
 
PsiI TTA'TAA

Cuts at:      0     139     564 
   Size:        139     425
 
StyI C'CwwG_G

Cuts at:      0     290     479     564 
   Size:        290     189      85

  Fragments arranged by size: 

                 290     189      85
 

 Enzymes that do cut:

     AcuI     BanI    BbvCI Bme1580I     BplI     BpmI   Bpu10I    BseRI
    BsmBI Bsp1286I    BsrBI    BsrDI    BssSI    BstXI     BtgI     BtsI
     DraI     EaeI     EarI     EciI  Eco57MI    Hin4I     MscI     MslI
     NcoI     NdeI     PsiI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI