(Linear) (Six-base) MAPSORT of: KIBB6830.seq  Check: 4194  from: 1  to: 567



KIBB6830.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      79     567 
   Size:         79     488
 
AlwNI CAG_nnn'CTG

Cuts at:      0     288     567 
   Size:        288     279
 
ApaLI G'TGCA_C

Cuts at:      0     137     567 
   Size:        137     430
 
AvaI C'yCGr_G

Cuts at:      0     530     567 
   Size:        530      37
 
BamHI G'GATC_C

Cuts at:      0     275     567 
   Size:        275     292
 
BbvCI CC'TCA_GC

Cuts at:      0     391     567 
   Size:        391     176
 
BglI GCCn_nnn'nGGC

Cuts at:      0     362     517     567 
   Size:        362     155      50

  Fragments arranged by size: 

                 362     155      50
 
Bme1580I G_kGCm'C

Cuts at:      0     141     567 
   Size:        141     426
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     394     432     567 
   Size:        394      38     135

  Fragments arranged by size: 

                 394     135      38
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     153     490     567 
   Size:        153     337      77

  Fragments arranged by size: 

                 337     153      77
 
Bpu10I CC'TnA_GC

Cuts at:      0      50     391     567 
   Size:         50     341     176

  Fragments arranged by size: 

                 341     176      50
 
BseYI C'CCAG_C

Cuts at:      0       3     454     567 
   Size:          3     451     113

  Fragments arranged by size: 

                 451     113       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     567 
   Size:        105     462
 
BsiHKAI G_wGCw'C

Cuts at:      0     141     567 
   Size:        141     426
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     478     567 
   Size:        478      89
 
Bsp1286I G_dGCh'C

Cuts at:      0     141     567 
   Size:        141     426
 
BstEII G'GTnAC_C

Cuts at:      0     415     567 
   Size:        415     152
 
BstYI r'GATC_y

Cuts at:      0     162     275     567 
   Size:        162     113     292

  Fragments arranged by size: 

                 292     162     113
 
Bsu36I CC'TnA_GG

Cuts at:      0     410     567 
   Size:        410     157
 
BtgI C'CryG_G

Cuts at:      0     463     543     567 
   Size:        463      80      24

  Fragments arranged by size: 

                 463      80      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     167     179     567 
   Size:        167      12     388

  Fragments arranged by size: 

                 388     167      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     153     490     567 
   Size:        153     337      77

  Fragments arranged by size: 

                 337     153      77
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     126     158     567 
   Size:        126      32     409

  Fragments arranged by size: 

                 409     126      32
 
NcoI C'CATG_G

Cuts at:      0     463     543     567 
   Size:        463      80      24

  Fragments arranged by size: 

                 463      80      24
 
PshAI GACnn'nnGTC

Cuts at:      0     254     567 
   Size:        254     313
 
PstI C_TGCA'G

Cuts at:      0     407     567 
   Size:        407     160
 
SfcI C'TryA_G

Cuts at:      0     403     521     567 
   Size:        403     118      46

  Fragments arranged by size: 

                 403     118      46
 
SmaI CCC'GGG

Cuts at:      0     532     567 
   Size:        532      35
 
SpeI A'CTAG_T

Cuts at:      0     147     567 
   Size:        147     420
 
StuI AGG'CCT

Cuts at:      0     511     567 
   Size:        511      56
 
StyI C'CwwG_G

Cuts at:      0     237     463     543     567 
   Size:        237     226      80      24

  Fragments arranged by size: 

                 237     226      80      24
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     214     220     567 
   Size:        214       6     347

  Fragments arranged by size: 

                 347     214       6
 
XmaI C'CCGG_G

Cuts at:      0     530     567 
   Size:        530      37
 

 Enzymes that do cut:

     AlfI    AlwNI    ApaLI     AvaI    BamHI    BbvCI     BglI Bme1580I
     BmrI     BpmI   Bpu10I    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I
   BstEII    BstYI   Bsu36I     BtgI     EarI  Eco57MI    Hin4I     NcoI
    PshAI     PstI     SfcI     SmaI     SpeI     StuI     StyI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SphI
     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmnI     ZraI