Restriction map for
pF1KB6831
(Linear) (Six-base) MAPSORT of: KIBB6831.seq Check: 8100 from: 1 to: 498
KIBB6831.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AleI CACnn'nnGTG
Cuts at: 0 34 498
Size: 34 464
AvaI C'yCGr_G
Cuts at: 0 72 103 498
Size: 72 31 395
Fragments arranged by size:
395 72 31
BanI G'GyrC_C
Cuts at: 0 381 498
Size: 381 117
BanII G_rGCy'C
Cuts at: 0 467 498
Size: 467 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 153 498
Size: 153 345
BclI T'GATC_A
Cuts at: 0 290 498
Size: 290 208
Bme1580I G_kGCm'C
Cuts at: 0 386 498
Size: 386 112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 98 498
Size: 98 400
BsaI GGTCTCn'nnnn_
Cuts at: 0 114 415 498
Size: 114 301 83
Fragments arranged by size:
301 114 83
BsaHI Gr'CG_yC
Cuts at: 0 78 498
Size: 78 420
BsaWI w'CCGG_w
Cuts at: 0 12 498
Size: 12 486
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 68 98 498
Size: 68 30 400
Fragments arranged by size:
400 68 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 208 241 244 253 498
Size: 76 132 33 3 9 245
Fragments arranged by size:
245 132 76 33 9 3
BseYI C'CCAG_C
Cuts at: 0 123 302 498
Size: 123 179 196
Fragments arranged by size:
196 179 123
BsiHKAI G_wGCw'C
Cuts at: 0 467 498
Size: 467 31
BsmBI CGTCTCn'nnnn_
Cuts at: 0 69 498
Size: 69 429
Bsp1286I G_dGCh'C
Cuts at: 0 386 467 498
Size: 386 81 31
Fragments arranged by size:
386 81 31
BspEI T'CCGG_A
Cuts at: 0 12 498
Size: 12 486
BspHI T'CATG_A
Cuts at: 0 455 498
Size: 455 43
BstXI CCAn_nnnn'nTGG
Cuts at: 0 159 498
Size: 159 339
EaeI y'GGCC_r
Cuts at: 0 434 498
Size: 434 64
EarI CTCTTCn'nnn_
Cuts at: 0 167 185 218 230 243 498
Size: 167 18 33 12 13 255
Fragments arranged by size:
255 167 33 18 13 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 418 498
Size: 418 80
EcoICRI GAG'CTC
Cuts at: 0 465 498
Size: 465 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 352 498
Size: 352 146
EcoO109I rG'GnC_Cy
Cuts at: 0 120 180 498
Size: 120 60 318
Fragments arranged by size:
318 120 60
HaeII r_GCGC'y
Cuts at: 0 309 498
Size: 309 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 68 100 498
Size: 68 32 398
Fragments arranged by size:
398 68 32
MscI TGG'CCA
Cuts at: 0 436 498
Size: 436 62
MslI CAynn'nnrTG
Cuts at: 0 34 498
Size: 34 464
MspA1I CmG'CkG
Cuts at: 0 369 498
Size: 369 129
PfoI T'CCnGG_A
Cuts at: 0 412 498
Size: 412 86
PpuMI rG'GwC_Cy
Cuts at: 0 120 180 498
Size: 120 60 318
Fragments arranged by size:
318 120 60
PvuII CAG'CTG
Cuts at: 0 369 498
Size: 369 129
SacI G_AGCT'C
Cuts at: 0 467 498
Size: 467 31
SanDI GG'GwC_CC
Cuts at: 0 120 180 498
Size: 120 60 318
Fragments arranged by size:
318 120 60
SmaI CCC'GGG
Cuts at: 0 105 498
Size: 105 393
StuI AGG'CCT
Cuts at: 0 358 498
Size: 358 140
StyI C'CwwG_G
Cuts at: 0 113 498
Size: 113 385
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 475 498
Size: 475 23
Tth111I GACn'n_nGTC
Cuts at: 0 46 498
Size: 46 452
XmaI C'CCGG_G
Cuts at: 0 103 498
Size: 103 395
Enzymes that do cut:
AleI AvaI BanI BanII BbsI BclI Bme1580I BplI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BstXI EaeI EarI EciI EcoICRI
EcoNI EcoO109I HaeII Hin4I MscI MslI MspA1I PfoI
PpuMI PvuII SacI SanDI SmaI StuI StyI TaqII
Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsgI
BsiEI BsiWI BsmI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI Eco57MI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI XbaI XcmI XhoI
XmnI ZraI