(Linear) (Six-base) MAPSORT of: KIBB6833.seq  Check: 7351  from: 1  to: 507



KIBB6833.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     507 
   Size:        184     323
 
AfeI AGC'GCT

Cuts at:      0     486     507 
   Size:        486      21
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      56      88     507 
   Size:         56      32     419

  Fragments arranged by size: 

                 419      56      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     291     507 
   Size:        291     216
 
BanI G'GyrC_C

Cuts at:      0     355     507 
   Size:        355     152
 
BanII G_rGCy'C

Cuts at:      0     467     507 
   Size:        467      40
 
Bme1580I G_kGCm'C

Cuts at:      0     429     494     507 
   Size:        429      65      13

  Fragments arranged by size: 

                 429      65      13
 
BmgBI CAC'GTC

Cuts at:      0     297     507 
   Size:        297     210
 
BsaHI Gr'CG_yC

Cuts at:      0     344     507 
   Size:        344     163
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     199     457     507 
   Size:        199     258      50

  Fragments arranged by size: 

                 258     199      50
 
BseYI C'CCAG_C

Cuts at:      0     473     487     507 
   Size:        473      14      20

  Fragments arranged by size: 

                 473      20      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33     118     193     507 
   Size:         33      85      75     314

  Fragments arranged by size: 

                 314      85      75      33
 
BsiEI CG_ry'CG

Cuts at:      0     237     341     507 
   Size:        237     104     166

  Fragments arranged by size: 

                 237     166     104
 
BsiHKAI G_wGCw'C

Cuts at:      0     203     467     507 
   Size:        203     264      40

  Fragments arranged by size: 

                 264     203      40
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     429     467     494     507 
   Size:        203     226      38      27      13

  Fragments arranged by size: 

                 226     203      38      27      13
 
BsrFI r'CCGG_y

Cuts at:      0      52     264     507 
   Size:         52     212     243

  Fragments arranged by size: 

                 243     212      52
 
BtgI C'CryG_G

Cuts at:      0      41     275     363     507 
   Size:         41     234      88     144

  Fragments arranged by size: 

                 234     144      88      41
 
EaeI y'GGCC_r

Cuts at:      0      77     234     272     338     507 
   Size:         77     157      38      66     169

  Fragments arranged by size: 

                 169     157      77      66      38
 
EagI C'GGCC_G

Cuts at:      0     234     338     507 
   Size:        234     104     169

  Fragments arranged by size: 

                 234     169     104
 
EarI CTCTTCn'nnn_

Cuts at:      0      40     507 
   Size:         40     467
 
EcoICRI GAG'CTC

Cuts at:      0     465     507 
   Size:        465      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     507 
   Size:        184     323
 
FspI TGC'GCA

Cuts at:      0     304     507 
   Size:        304     203
 
FspAI rTGC'GCAy

Cuts at:      0     304     507 
   Size:        304     203
 
HaeII r_GCGC'y

Cuts at:      0     132     163     319     488     507 
   Size:        132      31     156     169      19

  Fragments arranged by size: 

                 169     156     132      31      19
 
MscI TGG'CCA

Cuts at:      0     274     507 
   Size:        274     233
 
MspA1I CmG'CkG

Cuts at:      0      43     102     365     507 
   Size:         43      59     263     142

  Fragments arranged by size: 

                 263     142      59      43
 
NcoI C'CATG_G

Cuts at:      0     275     507 
   Size:        275     232
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     281     507 
   Size:        281     226
 
SacI G_AGCT'C

Cuts at:      0     467     507 
   Size:        467      40
 
SacII CC_GC'GG

Cuts at:      0      44     366     507 
   Size:         44     322     141

  Fragments arranged by size: 

                 322     141      44
 
SgrAI Cr'CCGG_yG

Cuts at:      0      52     507 
   Size:         52     455
 
StuI AGG'CCT

Cuts at:      0     178     507 
   Size:        178     329
 
StyI C'CwwG_G

Cuts at:      0     275     507 
   Size:        275     232
 
XmnI GAAnn'nnTTC

Cuts at:      0     145     507 
   Size:        145     362
 

 Enzymes that do cut:

     AcuI     AfeI     AloI    AlwNI     BanI    BanII Bme1580I    BmgBI
    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BsrFI
     BtgI     EaeI     EagI     EarI  EcoICRI  Eco57MI     FspI    FspAI
    HaeII     MscI   MspA1I     NcoI    PflMI     SacI    SacII    SgrAI
     StuI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI