Restriction map for
pF1KB6834
(Linear) (Six-base) MAPSORT of: KIBB6834.seq Check: 3359 from: 1 to: 567
KIBB6834.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 117 567
Size: 117 450
AflIII A'CryG_T
Cuts at: 0 470 567
Size: 470 97
AleI CACnn'nnGTG
Cuts at: 0 475 567
Size: 475 92
BanI G'GyrC_C
Cuts at: 0 28 567
Size: 28 539
BbeI G_GCGC'C
Cuts at: 0 32 567
Size: 32 535
BciVI GTATCCnnnnn_n'
Cuts at: 0 162 567
Size: 162 405
Bme1580I G_kGCm'C
Cuts at: 0 329 567
Size: 329 238
BmgBI CAC'GTC
Cuts at: 0 149 324 567
Size: 149 175 243
Fragments arranged by size:
243 175 149
Bpu10I CC'TnA_GC
Cuts at: 0 389 567
Size: 389 178
BsaI GGTCTCn'nnnn_
Cuts at: 0 421 567
Size: 421 146
BsaHI Gr'CG_yC
Cuts at: 0 29 567
Size: 29 538
BsaWI w'CCGG_w
Cuts at: 0 119 567
Size: 119 448
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 567
Size: 199 368
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 567
Size: 347 220
BsiHKAI G_wGCw'C
Cuts at: 0 563 567
Size: 563 4
BsmBI CGTCTCn'nnnn_
Cuts at: 0 139 567
Size: 139 428
Bsp1286I G_dGCh'C
Cuts at: 0 329 563 567
Size: 329 234 4
Fragments arranged by size:
329 234 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 117 567
Size: 117 450
BsrFI r'CCGG_y
Cuts at: 0 31 175 567
Size: 31 144 392
Fragments arranged by size:
392 144 31
BstAPI GCAn_nnn'nTGC
Cuts at: 0 501 548 567
Size: 501 47 19
Fragments arranged by size:
501 47 19
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 567
Size: 336 231
BstYI r'GATC_y
Cuts at: 0 485 567
Size: 485 82
BtgI C'CryG_G
Cuts at: 0 329 567
Size: 329 238
DraIII CAC_nnn'GTG
Cuts at: 0 84 567
Size: 84 483
EaeI y'GGCC_r
Cuts at: 0 177 434 567
Size: 177 257 133
Fragments arranged by size:
257 177 133
EcoO109I rG'GnC_Cy
Cuts at: 0 393 567
Size: 393 174
FspI TGC'GCA
Cuts at: 0 217 567
Size: 217 350
FspAI rTGC'GCAy
Cuts at: 0 217 567
Size: 217 350
HaeII r_GCGC'y
Cuts at: 0 32 197 567
Size: 32 165 370
Fragments arranged by size:
370 165 32
KasI G'GCGC_C
Cuts at: 0 28 567
Size: 28 539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 567
Size: 137 430
MslI CAynn'nnrTG
Cuts at: 0 475 567
Size: 475 92
MspA1I CmG'CkG
Cuts at: 0 66 567
Size: 66 501
NaeI GCC'GGC
Cuts at: 0 33 177 567
Size: 33 144 390
Fragments arranged by size:
390 144 33
NarI GG'CG_CC
Cuts at: 0 29 567
Size: 29 538
NcoI C'CATG_G
Cuts at: 0 329 567
Size: 329 238
NgoMIV G'CCGG_C
Cuts at: 0 31 175 567
Size: 31 144 392
Fragments arranged by size:
392 144 31
NspI r_CATG'y
Cuts at: 0 216 567
Size: 216 351
PflMI CCAn_nnn'nTGG
Cuts at: 0 84 567
Size: 84 483
PfoI T'CCnGG_A
Cuts at: 0 164 567
Size: 164 403
PpuMI rG'GwC_Cy
Cuts at: 0 393 567
Size: 393 174
PvuII CAG'CTG
Cuts at: 0 66 567
Size: 66 501
SfcI C'TryA_G
Cuts at: 0 104 567
Size: 104 463
SfoI GGC'GCC
Cuts at: 0 30 567
Size: 30 537
SgrAI Cr'CCGG_yG
Cuts at: 0 31 567
Size: 31 536
StyI C'CwwG_G
Cuts at: 0 329 567
Size: 329 238
TatI w'GTAC_w
Cuts at: 0 188 209 284 567
Size: 188 21 75 283
Fragments arranged by size:
283 188 75 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 336 368 567
Size: 336 32 199
Fragments arranged by size:
336 199 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 336 567
Size: 336 231
Enzymes that do cut:
AarI AflIII AleI BanI BbeI BciVI Bme1580I BmgBI
Bpu10I BsaI BsaHI BsaWI BseRI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrFI BstAPI BstXI BstYI BtgI DraIII
EaeI EcoO109I FspI FspAI HaeII KasI MmeI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PfoI
PpuMI PvuII SfcI SfoI SgrAI StyI TatI TstI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI BmrI
BmtI BplI BpmI BpuEI BsaAI BsaBI BsaXI BseYI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FseI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MscI NdeI NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI XmnI ZraI