(Linear) (Six-base) MAPSORT of: KIBB6835.seq  Check: 1770  from: 1  to: 510



KIBB6835.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AflIII A'CryG_T

Cuts at:      0     147     510 
   Size:        147     363
 
AlwNI CAG_nnn'CTG

Cuts at:      0      56     510 
   Size:         56     454
 
ApoI r'AATT_y

Cuts at:      0     428     510 
   Size:        428      82
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     480     510 
   Size:        480      30
 
BbvCI CC'TCA_GC

Cuts at:      0     215     510 
   Size:        215     295
 
BfrBI ATG'CAT

Cuts at:      0      32     123     510 
   Size:         32      91     387

  Fragments arranged by size: 

                 387      91      32
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     363     510 
   Size:        363     147
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     510 
   Size:         78     432
 
Bpu10I CC'TnA_GC

Cuts at:      0     215     510 
   Size:        215     295
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     256     510 
   Size:        256     254
 
BseYI C'CCAG_C

Cuts at:      0     210     510 
   Size:        210     300
 
BsiHKAI G_wGCw'C

Cuts at:      0      70     510 
   Size:         70     440
 
Bsp1286I G_dGCh'C

Cuts at:      0      70     510 
   Size:         70     440
 
BspHI T'CATG_A

Cuts at:      0     234     510 
   Size:        234     276
 
BsrDI GCAATG_nn'

Cuts at:      0     305     510 
   Size:        305     205
 
EarI CTCTTCn'nnn_

Cuts at:      0      74     233     510 
   Size:         74     159     277

  Fragments arranged by size: 

                 277     159      74
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     510 
   Size:         78     432
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     262     394     510 
   Size:        262     132     116

  Fragments arranged by size: 

                 262     132     116
 
HincII GTy'rAC

Cuts at:      0     305     510 
   Size:        305     205
 
MspA1I CmG'CkG

Cuts at:      0     214     453     510 
   Size:        214     239      57

  Fragments arranged by size: 

                 239     214      57
 
NsiI A_TGCA'T

Cuts at:      0      34     125     510 
   Size:         34      91     385

  Fragments arranged by size: 

                 385      91      34
 
NspI r_CATG'y

Cuts at:      0      36     151     510 
   Size:         36     115     359

  Fragments arranged by size: 

                 359     115      36
 
PciI A'CATG_T

Cuts at:      0     147     510 
   Size:        147     363
 
PfoI T'CCnGG_A

Cuts at:      0      55     365     510 
   Size:         55     310     145

  Fragments arranged by size: 

                 310     145      55
 
PvuII CAG'CTG

Cuts at:      0     214     453     510 
   Size:        214     239      57

  Fragments arranged by size: 

                 239     214      57
 
SphI G_CATG'C

Cuts at:      0      36     510 
   Size:         36     474
 
XmnI GAAnn'nnTTC

Cuts at:      0     374     510 
   Size:        374     136
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI     BbsI    BbvCI    BfrBI     BmrI     BpmI
   Bpu10I    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI    BsrDI     EarI
  Eco57MI     FalI   HincII   MspA1I     NsiI     NspI     PciI     PfoI
    PvuII     SphI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI