Restriction map for
pF1KB6840
(Linear) (Six-base) MAPSORT of: KIBB6840.seq Check: 877 from: 1 to: 573
KIBB6840.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 245 573
Size: 245 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 573
Size: 217 356
AflIII A'CryG_T
Cuts at: 0 239 573
Size: 239 334
AgeI A'CCGG_T
Cuts at: 0 505 573
Size: 505 68
AleI CACnn'nnGTG
Cuts at: 0 109 573
Size: 109 464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 226 573
Size: 226 347
AlwNI CAG_nnn'CTG
Cuts at: 0 549 573
Size: 549 24
ApaI G_GGCC'C
Cuts at: 0 461 573
Size: 461 112
ApoI r'AATT_y
Cuts at: 0 376 573
Size: 376 197
AvaI C'yCGr_G
Cuts at: 0 287 347 416 573
Size: 287 60 69 157
Fragments arranged by size:
287 157 69 60
BanI G'GyrC_C
Cuts at: 0 210 573
Size: 210 363
BanII G_rGCy'C
Cuts at: 0 48 461 573
Size: 48 413 112
Fragments arranged by size:
413 112 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 387 573
Size: 387 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 32 66 96 130 573
Size: 32 34 30 34 443
Fragments arranged by size:
443 34 34 32 30
Bme1580I G_kGCm'C
Cuts at: 0 215 461 573
Size: 215 246 112
Fragments arranged by size:
246 215 112
BmgBI CAC'GTC
Cuts at: 0 357 573
Size: 357 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 210 573
Size: 210 363
BsaI GGTCTCn'nnnn_
Cuts at: 0 163 573
Size: 163 410
BsaWI w'CCGG_w
Cuts at: 0 505 573
Size: 505 68
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 573
Size: 391 30 152
Fragments arranged by size:
391 152 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 460 505 573
Size: 205 255 45 68
Fragments arranged by size:
255 205 68 45
BsiHKAI G_wGCw'C
Cuts at: 0 310 573
Size: 310 263
Bsp1286I G_dGCh'C
Cuts at: 0 48 215 310 461 573
Size: 48 167 95 151 112
Fragments arranged by size:
167 151 112 95 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 245 381 573
Size: 245 136 192
Fragments arranged by size:
245 192 136
BsrFI r'CCGG_y
Cuts at: 0 250 505 573
Size: 250 255 68
Fragments arranged by size:
255 250 68
BssSI C'ACGA_G
Cuts at: 0 41 573
Size: 41 532
BstXI CCAn_nnnn'nTGG
Cuts at: 0 230 573
Size: 230 343
BtgI C'CryG_G
Cuts at: 0 65 527 573
Size: 65 462 46
Fragments arranged by size:
462 65 46
BtsI GCAGTG_nn'
Cuts at: 0 310 573
Size: 310 263
EarI CTCTTCn'nnn_
Cuts at: 0 478 487 529 573
Size: 478 9 42 44
Fragments arranged by size:
478 44 42 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 573
Size: 217 356
EcoO109I rG'GnC_Cy
Cuts at: 0 458 465 573
Size: 458 7 108
Fragments arranged by size:
458 108 7
HaeII r_GCGC'y
Cuts at: 0 450 573
Size: 450 123
HincII GTy'rAC
Cuts at: 0 237 573
Size: 237 336
MslI CAynn'nnrTG
Cuts at: 0 70 109 228 573
Size: 70 39 119 345
Fragments arranged by size:
345 119 70 39
MspA1I CmG'CkG
Cuts at: 0 247 573
Size: 247 326
NaeI GCC'GGC
Cuts at: 0 252 573
Size: 252 321
NcoI C'CATG_G
Cuts at: 0 65 527 573
Size: 65 462 46
Fragments arranged by size:
462 65 46
NgoMIV G'CCGG_C
Cuts at: 0 250 573
Size: 250 323
PspOMI G'GGCC_C
Cuts at: 0 457 573
Size: 457 116
PvuII CAG'CTG
Cuts at: 0 247 573
Size: 247 326
SapI GCTCTTCn'nnn_
Cuts at: 0 529 573
Size: 529 44
SmaI CCC'GGG
Cuts at: 0 349 573
Size: 349 224
SrfI GCCC'GGGC
Cuts at: 0 349 573
Size: 349 224
StyI C'CwwG_G
Cuts at: 0 65 527 573
Size: 65 462 46
Fragments arranged by size:
462 65 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 46 573
Size: 46 527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 139 171 573
Size: 139 32 402
Fragments arranged by size:
402 139 32
XmaI C'CCGG_G
Cuts at: 0 347 573
Size: 347 226
Enzymes that do cut:
AarI AcuI AflIII AgeI AleI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BcgI Bme1580I BmgBI
BmrI BsaI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspMI
BsrFI BssSI BstXI BtgI BtsI EarI Eco57MI EcoO109I
HaeII HincII MslI MspA1I NaeI NcoI NgoMIV PspOMI
PvuII SapI SmaI SrfI StyI TaqII TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BciVI BclI BfrBI BglI BglII BlpI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NarI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SspI StuI SwaI TatI Tth111I XbaI
XcmI XhoI XmnI ZraI