(Linear) (Six-base) MAPSORT of: KIBB6845.seq  Check: 6171  from: 1  to: 525



KIBB6845.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

BamHI G'GATC_C

Cuts at:      0     110     253     525 
   Size:        110     143     272

  Fragments arranged by size: 

                 272     143     110
 
BanI G'GyrC_C

Cuts at:      0     334     525 
   Size:        334     191
 
BanII G_rGCy'C

Cuts at:      0     317     344     525 
   Size:        317      27     181

  Fragments arranged by size: 

                 317     181      27
 
BbvCI CC'TCA_GC

Cuts at:      0     248     525 
   Size:        248     277
 
BglII A'GATC_T

Cuts at:      0     192     525 
   Size:        192     333
 
Bme1580I G_kGCm'C

Cuts at:      0     337     525 
   Size:        337     188
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      17     525 
   Size:         17     508
 
Bpu10I CC'TnA_GC

Cuts at:      0     248     525 
   Size:        248     277
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     525 
   Size:        405     120
 
BsaBI GATnn'nnATC

Cuts at:      0     191     525 
   Size:        191     334
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     357     387     525 
   Size:        357      30     138

  Fragments arranged by size: 

                 357     138      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     525 
   Size:         89     436
 
BsiHKAI G_wGCw'C

Cuts at:      0     239     525 
   Size:        239     286
 
Bsp1286I G_dGCh'C

Cuts at:      0     239     317     337     344     525 
   Size:        239      78      20       7     181

  Fragments arranged by size: 

                 239     181      78      20       7
 
BstEII G'GTnAC_C

Cuts at:      0     497     525 
   Size:        497      28
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     179     525 
   Size:        179     346
 
BstYI r'GATC_y

Cuts at:      0     110     192     253     525 
   Size:        110      82      61     272

  Fragments arranged by size: 

                 272     110      82      61
 
BtgI C'CryG_G

Cuts at:      0      11     525 
   Size:         11     514
 
BtsI GCAGTG_nn'

Cuts at:      0     147     378     525 
   Size:        147     231     147

  Fragments arranged by size: 

                 231     147     147
 
EaeI y'GGCC_r

Cuts at:      0     429     525 
   Size:        429      96
 
EarI CTCTTCn'nnn_

Cuts at:      0     271     525 
   Size:        271     254
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     102     134     525 
   Size:        102      32     391

  Fragments arranged by size: 

                 391     102      32
 
MslI CAynn'nnrTG

Cuts at:      0     152     525 
   Size:        152     373
 
MspA1I CmG'CkG

Cuts at:      0     116     525 
   Size:        116     409
 
NcoI C'CATG_G

Cuts at:      0      11     525 
   Size:         11     514
 
PfoI T'CCnGG_A

Cuts at:      0     178     525 
   Size:        178     347
 
PsiI TTA'TAA

Cuts at:      0     482     525 
   Size:        482      43
 
SapI GCTCTTCn'nnn_

Cuts at:      0     271     525 
   Size:        271     254
 
SexAI A'CCwGG_T

Cuts at:      0     224     525 
   Size:        224     301
 
SmlI C'TyrA_G

Cuts at:      0     420     525 
   Size:        420     105
 
StuI AGG'CCT

Cuts at:      0     136     525 
   Size:        136     389
 
StyI C'CwwG_G

Cuts at:      0      11     131     525 
   Size:         11     120     394

  Fragments arranged by size: 

                 394     120      11
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     212     244     245     277     357     389     525 
   Size:        212      32       1      32      80      32     136

  Fragments arranged by size: 

                 212     136      80      32      32      32       1
 

 Enzymes that do cut:

    BamHI     BanI    BanII    BbvCI    BglII Bme1580I     BmrI   Bpu10I
    BpuEI    BsaBI    BsaXI     BsgI  BsiHKAI Bsp1286I   BstEII    BstXI
    BstYI     BtgI     BtsI     EaeI     EarI    Hin4I     MslI   MspA1I
     NcoI     PfoI     PsiI     SapI    SexAI     SmlI     StuI     StyI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI