Restriction map for
pF1KB6846
(Linear) (Six-base) MAPSORT of: KIBB6846.seq Check: 9913 from: 1 to: 579
KIBB6846.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 0 512 579
Size: 512 67
Acc65I G'GTAC_C
Cuts at: 0 122 579
Size: 122 457
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 579
Size: 559 20
AlwNI CAG_nnn'CTG
Cuts at: 0 279 318 579
Size: 279 39 261
Fragments arranged by size:
279 261 39
ApaLI G'TGCA_C
Cuts at: 0 7 579
Size: 7 572
BanI G'GyrC_C
Cuts at: 0 79 122 579
Size: 79 43 457
Fragments arranged by size:
457 79 43
BanII G_rGCy'C
Cuts at: 0 350 414 423 579
Size: 350 64 9 156
Fragments arranged by size:
350 156 64 9
BbeI G_GCGC'C
Cuts at: 0 83 579
Size: 83 496
BbvCI CC'TCA_GC
Cuts at: 0 456 579
Size: 456 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1 35 579
Size: 1 34 544
Fragments arranged by size:
544 34 1
BglI GCCn_nnn'nGGC
Cuts at: 0 142 579
Size: 142 437
Bme1580I G_kGCm'C
Cuts at: 0 11 579
Size: 11 568
BmrI ACTGGGnnnn_n'
Cuts at: 0 64 579
Size: 64 515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 428 579
Size: 136 292 151
Fragments arranged by size:
292 151 136
Bpu10I CC'TnA_GC
Cuts at: 0 456 579
Size: 456 123
BsaHI Gr'CG_yC
Cuts at: 0 80 579
Size: 80 499
BseYI C'CCAG_C
Cuts at: 0 335 579
Size: 335 244
BsiHKAI G_wGCw'C
Cuts at: 0 11 579
Size: 11 568
Bsp1286I G_dGCh'C
Cuts at: 0 11 350 414 423 579
Size: 11 339 64 9 156
Fragments arranged by size:
339 156 64 11 9
BsrGI T'GTAC_A
Cuts at: 0 545 579
Size: 545 34
BtgI C'CryG_G
Cuts at: 0 340 579
Size: 340 239
BtsI GCAGTG_nn'
Cuts at: 0 174 479 579
Size: 174 305 100
Fragments arranged by size:
305 174 100
DrdI GACnn_nn'nnGTC
Cuts at: 0 109 579
Size: 109 470
EarI CTCTTCn'nnn_
Cuts at: 0 229 336 508 579
Size: 229 107 172 71
Fragments arranged by size:
229 172 107 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 428 559 579
Size: 136 292 131 20
Fragments arranged by size:
292 136 131 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 130 234 579
Size: 130 104 345
Fragments arranged by size:
345 130 104
EcoO109I rG'GnC_Cy
Cuts at: 0 276 315 579
Size: 276 39 264
Fragments arranged by size:
276 264 39
HaeII r_GCGC'y
Cuts at: 0 83 579
Size: 83 496
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 579
Size: 439 32 108
Fragments arranged by size:
439 108 32
HincII GTy'rAC
Cuts at: 0 327 579
Size: 327 252
KasI G'GCGC_C
Cuts at: 0 79 579
Size: 79 500
KpnI G_GTAC'C
Cuts at: 0 126 579
Size: 126 453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 579
Size: 272 307
MspA1I CmG'CkG
Cuts at: 0 357 460 579
Size: 357 103 119
Fragments arranged by size:
357 119 103
NarI GG'CG_CC
Cuts at: 0 80 579
Size: 80 499
NspI r_CATG'y
Cuts at: 0 364 579
Size: 364 215
PfoI T'CCnGG_A
Cuts at: 0 113 579
Size: 113 466
PstI C_TGCA'G
Cuts at: 0 181 356 565 579
Size: 181 175 209 14
Fragments arranged by size:
209 181 175 14
PvuII CAG'CTG
Cuts at: 0 357 460 579
Size: 357 103 119
Fragments arranged by size:
357 119 103
SbfI CC_TGCA'GG
Cuts at: 0 565 579
Size: 565 14
SfcI C'TryA_G
Cuts at: 0 177 352 561 579
Size: 177 175 209 18
Fragments arranged by size:
209 177 175 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 142 579
Size: 142 437
SfoI GGC'GCC
Cuts at: 0 81 579
Size: 81 498
StuI AGG'CCT
Cuts at: 0 559 579
Size: 559 20
StyI C'CwwG_G
Cuts at: 0 137 579
Size: 137 442
TatI w'GTAC_w
Cuts at: 0 545 579
Size: 545 34
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApaLI BanI BanII BbeI
BbvCI BcgI BglI Bme1580I BmrI BpmI Bpu10I BsaHI
BseYI BsiHKAI Bsp1286I BsrGI BtgI BtsI DrdI EarI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI KpnI
MmeI MspA1I NarI NspI PfoI PstI PvuII SbfI
SfcI SfiI SfoI StuI StyI TatI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EaeI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI MfeI MluI MscI MslI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI