Restriction map for
pF1KB6847
(Linear) (Six-base) MAPSORT of: KIBB6847.seq Check: 3047 from: 1 to: 528
KIBB6847.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 186 528
Size: 186 342
AccI GT'mk_AC
Cuts at: 0 185 528
Size: 185 343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 528
Size: 155 373
AflIII A'CryG_T
Cuts at: 0 275 528
Size: 275 253
AlwNI CAG_nnn'CTG
Cuts at: 0 313 528
Size: 313 215
AvaI C'yCGr_G
Cuts at: 0 9 356 528
Size: 9 347 172
Fragments arranged by size:
347 172 9
BanI G'GyrC_C
Cuts at: 0 265 340 347 528
Size: 265 75 7 181
Fragments arranged by size:
265 181 75 7
BanII G_rGCy'C
Cuts at: 0 363 528
Size: 363 165
BbsI GAAGACnn'nnnn_
Cuts at: 0 161 528
Size: 161 367
Bme1580I G_kGCm'C
Cuts at: 0 16 345 528
Size: 16 329 183
Fragments arranged by size:
329 183 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 315 528
Size: 315 213
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 403 528
Size: 403 125
BsiEI CG_ry'CG
Cuts at: 0 418 528
Size: 418 110
Bsp1286I G_dGCh'C
Cuts at: 0 16 345 363 528
Size: 16 329 18 165
Fragments arranged by size:
329 165 18 16
BspHI T'CATG_A
Cuts at: 0 377 528
Size: 377 151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 186 528
Size: 186 342
BsrBI CCG'CTC
Cuts at: 0 263 528
Size: 263 265
BsrFI r'CCGG_y
Cuts at: 0 413 417 528
Size: 413 4 111
Fragments arranged by size:
413 111 4
BstYI r'GATC_y
Cuts at: 0 236 528
Size: 236 292
DraIII CAC_nnn'GTG
Cuts at: 0 323 528
Size: 323 205
EaeI y'GGCC_r
Cuts at: 0 398 415 528
Size: 398 17 113
Fragments arranged by size:
398 113 17
EagI C'GGCC_G
Cuts at: 0 415 528
Size: 415 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 528
Size: 155 373
EcoO109I rG'GnC_Cy
Cuts at: 0 294 471 528
Size: 294 177 57
Fragments arranged by size:
294 177 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 144 513 528
Size: 144 369 15
Fragments arranged by size:
369 144 15
HaeII r_GCGC'y
Cuts at: 0 314 528
Size: 314 214
MslI CAynn'nnrTG
Cuts at: 0 382 528
Size: 382 146
MspA1I CmG'CkG
Cuts at: 0 459 528
Size: 459 69
NaeI GCC'GGC
Cuts at: 0 415 419 528
Size: 415 4 109
Fragments arranged by size:
415 109 4
NgoMIV G'CCGG_C
Cuts at: 0 413 417 528
Size: 413 4 111
Fragments arranged by size:
413 111 4
NspI r_CATG'y
Cuts at: 0 90 279 528
Size: 90 189 249
Fragments arranged by size:
249 189 90
PciI A'CATG_T
Cuts at: 0 275 528
Size: 275 253
PfoI T'CCnGG_A
Cuts at: 0 256 528
Size: 256 272
PpuMI rG'GwC_Cy
Cuts at: 0 294 471 528
Size: 294 177 57
Fragments arranged by size:
294 177 57
SphI G_CATG'C
Cuts at: 0 90 528
Size: 90 438
StuI AGG'CCT
Cuts at: 0 7 528
Size: 7 521
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 283 328 334 528
Size: 283 45 6 194
Fragments arranged by size:
283 194 45 6
TatI w'GTAC_w
Cuts at: 0 26 528
Size: 26 502
XmnI GAAnn'nnTTC
Cuts at: 0 229 528
Size: 229 299
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI AvaI BanI BanII
BbsI Bme1580I BmrI BseRI BsiEI Bsp1286I BspHI BspMI
BsrBI BsrFI BstYI DraIII EaeI EagI Eco57MI EcoO109I
FalI HaeII MslI MspA1I NaeI NgoMIV NspI PciI
PfoI PpuMI SphI StuI TaqII TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StyI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI