Restriction map for
pF1KB6848
(Linear) (Six-base) MAPSORT of: KIBB6848.seq Check: 2868 from: 1 to: 582
KIBB6848.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 582
Size: 179 403
AfeI AGC'GCT
Cuts at: 0 306 582
Size: 306 276
AleI CACnn'nnGTG
Cuts at: 0 400 582
Size: 400 182
AvaI C'yCGr_G
Cuts at: 0 29 582
Size: 29 553
BanII G_rGCy'C
Cuts at: 0 21 141 582
Size: 21 120 441
Fragments arranged by size:
441 120 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 268 392 582
Size: 268 124 190
Fragments arranged by size:
268 190 124
BbvCI CC'TCA_GC
Cuts at: 0 54 582
Size: 54 528
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 249 283 582
Size: 249 34 299
Fragments arranged by size:
299 249 34
BfrBI ATG'CAT
Cuts at: 0 3 582
Size: 3 579
BglI GCCn_nnn'nGGC
Cuts at: 0 190 582
Size: 190 392
Bme1580I G_kGCm'C
Cuts at: 0 53 582
Size: 53 529
BmgBI CAC'GTC
Cuts at: 0 239 582
Size: 239 343
Bpu10I CC'TnA_GC
Cuts at: 0 54 582
Size: 54 528
BsaWI w'CCGG_w
Cuts at: 0 275 582
Size: 275 307
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 257 287 582
Size: 257 30 295
Fragments arranged by size:
295 257 30
BsiEI CG_ry'CG
Cuts at: 0 259 582
Size: 259 323
BsiHKAI G_wGCw'C
Cuts at: 0 21 582
Size: 21 561
Bsp1286I G_dGCh'C
Cuts at: 0 21 53 141 582
Size: 21 32 88 441
Fragments arranged by size:
441 88 32 21
BspEI T'CCGG_A
Cuts at: 0 275 582
Size: 275 307
BstYI r'GATC_y
Cuts at: 0 230 582
Size: 230 352
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 147 582
Size: 147 435
BtsI GCAGTG_nn'
Cuts at: 0 200 265 582
Size: 200 65 317
Fragments arranged by size:
317 200 65
DraI TTT'AAA
Cuts at: 0 179 582
Size: 179 403
DrdI GACnn_nn'nnGTC
Cuts at: 0 363 582
Size: 363 219
EaeI y'GGCC_r
Cuts at: 0 256 476 582
Size: 256 220 106
Fragments arranged by size:
256 220 106
EagI C'GGCC_G
Cuts at: 0 256 582
Size: 256 326
EarI CTCTTCn'nnn_
Cuts at: 0 295 582
Size: 295 287
EcoICRI GAG'CTC
Cuts at: 0 19 582
Size: 19 563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 582
Size: 179 403
HaeII r_GCGC'y
Cuts at: 0 308 582
Size: 308 274
HincII GTy'rAC
Cuts at: 0 486 582
Size: 486 96
HindIII A'AGCT_T
Cuts at: 0 322 582
Size: 322 260
MscI TGG'CCA
Cuts at: 0 478 582
Size: 478 104
MslI CAynn'nnrTG
Cuts at: 0 400 472 582
Size: 400 72 110
Fragments arranged by size:
400 110 72
MspA1I CmG'CkG
Cuts at: 0 354 582
Size: 354 228
NdeI CA'TA_TG
Cuts at: 0 522 582
Size: 522 60
NsiI A_TGCA'T
Cuts at: 0 5 582
Size: 5 577
PflMI CCAn_nnn'nTGG
Cuts at: 0 13 582
Size: 13 569
SacI G_AGCT'C
Cuts at: 0 21 582
Size: 21 561
SapI GCTCTTCn'nnn_
Cuts at: 0 295 582
Size: 295 287
SmaI CCC'GGG
Cuts at: 0 31 582
Size: 31 551
StyI C'CwwG_G
Cuts at: 0 479 582
Size: 479 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 258 582
Size: 258 324
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 233 257 265 289 582
Size: 233 24 8 24 293
Fragments arranged by size:
293 233 24 24 8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 72 582
Size: 72 510
XmaI C'CCGG_G
Cuts at: 0 29 582
Size: 29 553
Enzymes that do cut:
AcuI AfeI AleI AvaI BanII BbsI BbvCI BcgI
BfrBI BglI Bme1580I BmgBI Bpu10I BsaWI BsaXI BsiEI
BsiHKAI Bsp1286I BspEI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EcoICRI Eco57MI HaeII HincII HindIII
MscI MslI MspA1I NdeI NsiI PflMI SacI SapI
SmaI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BciVI
BclI BglII BlpI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiWI
BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI