(Linear) (Six-base) MAPSORT of: KIBB6849.seq  Check: 6233  from: 1  to: 531



KIBB6849.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AleI CACnn'nnGTG

Cuts at:      0      82     265     531 
   Size:         82     183     266

  Fragments arranged by size: 

                 266     183      82
 
AlwNI CAG_nnn'CTG

Cuts at:      0      42      54     531 
   Size:         42      12     477

  Fragments arranged by size: 

                 477      42      12
 
ApaLI G'TGCA_C

Cuts at:      0      75     531 
   Size:         75     456
 
ApoI r'AATT_y

Cuts at:      0     426     531 
   Size:        426     105
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     531 
   Size:         10     521
 
Bme1580I G_kGCm'C

Cuts at:      0      79     531 
   Size:         79     452
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204     531 
   Size:        204     327
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      92     486     531 
   Size:         92     394      45

  Fragments arranged by size: 

                 394      92      45
 
BseYI C'CCAG_C

Cuts at:      0     139     299     531 
   Size:        139     160     232

  Fragments arranged by size: 

                 232     160     139
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     418     531 
   Size:        289     129     113

  Fragments arranged by size: 

                 289     129     113
 
BsiHKAI G_wGCw'C

Cuts at:      0      79     531 
   Size:         79     452
 
Bsp1286I G_dGCh'C

Cuts at:      0      79     531 
   Size:         79     452
 
BspHI T'CATG_A

Cuts at:      0     200     531 
   Size:        200     331
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     110     531 
   Size:        110     421
 
BsrFI r'CCGG_y

Cuts at:      0     157     531 
   Size:        157     374
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     147     531 
   Size:        147     384
 
BstYI r'GATC_y

Cuts at:      0     215     531 
   Size:        215     316
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     239     531 
   Size:        239     292
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204     531 
   Size:        204     327
 
HaeII r_GCGC'y

Cuts at:      0      43     531 
   Size:         43     488
 
MslI CAynn'nnrTG

Cuts at:      0      82     265     531 
   Size:         82     183     266

  Fragments arranged by size: 

                 266     183      82
 
PstI C_TGCA'G

Cuts at:      0     107     531 
   Size:        107     424
 
SfcI C'TryA_G

Cuts at:      0     103     531 
   Size:        103     428
 
StyI C'CwwG_G

Cuts at:      0     210     531 
   Size:        210     321
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      72     451     531 
   Size:         72     379      80

  Fragments arranged by size: 

                 379      80      72
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     399     531 
   Size:        399     132
 

 Enzymes that do cut:

     AleI    AlwNI    ApaLI     ApoI     BbsI Bme1580I     BpmI     BsaI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrFI    BstXI
    BstYI    BtgZI  Eco57MI    HaeII     MslI     PstI     SfcI     StyI
    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI