(Linear) (Six-base) MAPSORT of: KIBB6850.seq  Check: 6219  from: 1  to: 582



KIBB6850.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     320     582 
   Size:        293      27     262

  Fragments arranged by size: 

                 293     262      27
 
AlwNI CAG_nnn'CTG

Cuts at:      0     520     582 
   Size:        520      62
 
ApaLI G'TGCA_C

Cuts at:      0     103     582 
   Size:        103     479
 
BamHI G'GATC_C

Cuts at:      0     396     582 
   Size:        396     186
 
BanI G'GyrC_C

Cuts at:      0      51     582 
   Size:         51     531
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     184     582 
   Size:        184     398
 
BglII A'GATC_T

Cuts at:      0     489     582 
   Size:        489      93
 
Bme1580I G_kGCm'C

Cuts at:      0     107     582 
   Size:        107     475
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     497     582 
   Size:        497      85
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     582 
   Size:        517      65
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     160     582 
   Size:        160     422
 
BsiHKAI G_wGCw'C

Cuts at:      0     107     294     582 
   Size:        107     187     288

  Fragments arranged by size: 

                 288     187     107
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     294     582 
   Size:        107     187     288

  Fragments arranged by size: 

                 288     187     107
 
BsrGI T'GTAC_A

Cuts at:      0     111     582 
   Size:        111     471
 
BstYI r'GATC_y

Cuts at:      0     396     489     582 
   Size:        396      93      93

  Fragments arranged by size: 

                 396      93      93
 
DraI TTT'AAA

Cuts at:      0     266     582 
   Size:        266     316
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     320     497     582 
   Size:        293      27     177      85

  Fragments arranged by size: 

                 293     177      85      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      46     582 
   Size:         46     536
 
EcoRV GAT'ATC

Cuts at:      0     415     470     582 
   Size:        415      55     112

  Fragments arranged by size: 

                 415     112      55
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     550     582 
   Size:        550      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     582 
   Size:        201     381
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      99     582 
   Size:         99     483
 
SfcI C'TryA_G

Cuts at:      0     134     349     386     542     582 
   Size:        134     215      37     156      40

  Fragments arranged by size: 

                 215     156     134      40      37
 
SmlI C'TyrA_G

Cuts at:      0     496     582 
   Size:        496      86
 
SspI AAT'ATT

Cuts at:      0     201     582 
   Size:        201     381
 
StuI AGG'CCT

Cuts at:      0     233     582 
   Size:        233     349
 
StyI C'CwwG_G

Cuts at:      0      47     392     582 
   Size:         47     345     190

  Fragments arranged by size: 

                 345     190      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     424     582 
   Size:        424     158
 
TatI w'GTAC_w

Cuts at:      0     111     331     582 
   Size:        111     220     251

  Fragments arranged by size: 

                 251     220     111
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     158     190     582 
   Size:        158      32     392

  Fragments arranged by size: 

                 392     158      32
 

 Enzymes that do cut:

     AcuI    AlwNI    ApaLI    BamHI     BanI    BciVI    BglII Bme1580I
     BpmI    BpuEI    BseRI  BsiHKAI Bsp1286I    BsrGI    BstYI     DraI
  Eco57MI    EcoNI    EcoRV    Hin4I     MmeI    PflMI     SfcI     SmlI
     SspI     StuI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI