(Linear) (Six-base) MAPSORT of: KIBB6851.seq Check: 1056 from: 1 to: 399
KIBB6851.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
ApoI r'AATT_y
Cuts at: 0 187 374 399
Size: 187 187 25
Fragments arranged by size:
187 187 25
Bme1580I G_kGCm'C
Cuts at: 0 388 399
Size: 388 11
Bsp1286I G_dGCh'C
Cuts at: 0 388 399
Size: 388 11
ClaI AT'CG_AT
Cuts at: 0 134 399
Size: 134 265
DraI TTT'AAA
Cuts at: 0 201 299 399
Size: 201 98 100
Fragments arranged by size:
201 100 98
MfeI C'AATT_G
Cuts at: 0 171 399
Size: 171 228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 399
Size: 346 53
MslI CAynn'nnrTG
Cuts at: 0 212 399
Size: 212 187
MspA1I CmG'CkG
Cuts at: 0 247 399
Size: 247 152
PasI CC'CwG_GG
Cuts at: 0 252 399
Size: 252 147
PvuII CAG'CTG
Cuts at: 0 247 399
Size: 247 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 244 399
Size: 244 155
Enzymes that do cut:
ApoI Bme1580I Bsp1286I ClaI DraI MfeI MmeI MslI
MspA1I PasI PvuII TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI