(Linear) (Six-base) MAPSORT of: KIBB6852.seq  Check: 6306  from: 1  to: 582



KIBB6852.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      78      84     582 
   Size:         78       6     498

  Fragments arranged by size: 

                 498      78       6
 
AflII C'TTAA_G

Cuts at:      0     213     582 
   Size:        213     369
 
ApoI r'AATT_y

Cuts at:      0     160     582 
   Size:        160     422
 
BanI G'GyrC_C

Cuts at:      0       3      60      66     582 
   Size:          3      57       6     516

  Fragments arranged by size: 

                 516      57       6       3
 
BanII G_rGCy'C

Cuts at:      0     243     582 
   Size:        243     339
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     395     533     582 
   Size:        395     138      49

  Fragments arranged by size: 

                 395     138      49
 
BglI GCCn_nnn'nGGC

Cuts at:      0     514     582 
   Size:        514      68
 
Bme1580I G_kGCm'C

Cuts at:      0     250     582 
   Size:        250     332
 
BmgBI CAC'GTC

Cuts at:      0      18     582 
   Size:         18     564
 
BseYI C'CCAG_C

Cuts at:      0      51      87     292     582 
   Size:         51      36     205     290

  Fragments arranged by size: 

                 290     205      51      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56     582 
   Size:         56     526
 
BsiHKAI G_wGCw'C

Cuts at:      0     243     582 
   Size:        243     339
 
BsmI GAATG_Cn'

Cuts at:      0     315     582 
   Size:        315     267
 
Bsp1286I G_dGCh'C

Cuts at:      0     243     250     582 
   Size:        243       7     332

  Fragments arranged by size: 

                 332     243       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      78      84     508     582 
   Size:         78       6     424      74

  Fragments arranged by size: 

                 424      78      74       6
 
BsrDI GCAATG_nn'

Cuts at:      0     303     435     582 
   Size:        303     132     147

  Fragments arranged by size: 

                 303     147     132
 
BsrFI r'CCGG_y

Cuts at:      0      63     582 
   Size:         63     519
 
EarI CTCTTCn'nnn_

Cuts at:      0     296     582 
   Size:        296     286
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     445     582 
   Size:        445     137
 
EcoICRI GAG'CTC

Cuts at:      0     241     582 
   Size:        241     341
 
EcoRI G'AATT_C

Cuts at:      0     160     582 
   Size:        160     422
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     228     582 
   Size:        228     354
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     504     536     582 
   Size:        504      32      46

  Fragments arranged by size: 

                 504      46      32
 
HindIII A'AGCT_T

Cuts at:      0     554     582 
   Size:        554      28
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     582 
   Size:        386     196
 
MslI CAynn'nnrTG

Cuts at:      0     439     582 
   Size:        439     143
 
PasI CC'CwG_GG

Cuts at:      0     474     582 
   Size:        474     108
 
SacI G_AGCT'C

Cuts at:      0     243     582 
   Size:        243     339
 
SapI GCTCTTCn'nnn_

Cuts at:      0     296     582 
   Size:        296     286
 
SmlI C'TyrA_G

Cuts at:      0     213     582 
   Size:        213     369
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     433     466     582 
   Size:        433      33     116

  Fragments arranged by size: 

                 433     116      33
 
XmnI GAAnn'nnTTC

Cuts at:      0     335     582 
   Size:        335     247
 

 Enzymes that do cut:

     AarI    AflII     ApoI     BanI    BanII     BbsI     BglI Bme1580I
    BmgBI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI
    BsrFI     EarI     EciI  EcoICRI    EcoRI     FalI    Hin4I  HindIII
     MmeI     MslI     PasI     SacI     SapI     SmlI    TaqII     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI  Eco57MI    EcoNI EcoO109I
    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI