Restriction map for
pF1KB6854
(Linear) (Six-base) MAPSORT of: KIBB6854.seq Check: 7108 from: 1 to: 582
KIBB6854.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 408 582
Size: 408 174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 319 582
Size: 106 213 263
Fragments arranged by size:
263 213 106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 244 271 582
Size: 244 27 311
Fragments arranged by size:
311 244 27
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 418 450 582
Size: 418 32 132
Fragments arranged by size:
418 132 32
ApoI r'AATT_y
Cuts at: 0 139 582
Size: 139 443
AvaI C'yCGr_G
Cuts at: 0 170 507 582
Size: 170 337 75
Fragments arranged by size:
337 170 75
BanII G_rGCy'C
Cuts at: 0 110 582
Size: 110 472
BbsI GAAGACnn'nnnn_
Cuts at: 0 333 582
Size: 333 249
BglI GCCn_nnn'nGGC
Cuts at: 0 509 582
Size: 509 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 581 582
Size: 150 431 1
Fragments arranged by size:
431 150 1
BsaI GGTCTCn'nnnn_
Cuts at: 0 45 582
Size: 45 537
BsaHI Gr'CG_yC
Cuts at: 0 207 324 582
Size: 207 117 258
Fragments arranged by size:
258 207 117
BsaWI w'CCGG_w
Cuts at: 0 110 582
Size: 110 472
BseYI C'CCAG_C
Cuts at: 0 17 348 553 582
Size: 17 331 205 29
Fragments arranged by size:
331 205 29 17
BsmI GAATG_Cn'
Cuts at: 0 534 582
Size: 534 48
BsmBI CGTCTCn'nnnn_
Cuts at: 0 76 315 557 582
Size: 76 239 242 25
Fragments arranged by size:
242 239 76 25
Bsp1286I G_dGCh'C
Cuts at: 0 110 582
Size: 110 472
BspEI T'CCGG_A
Cuts at: 0 110 582
Size: 110 472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 408 582
Size: 408 174
BstBI TT'CG_AA
Cuts at: 0 215 582
Size: 215 367
BstYI r'GATC_y
Cuts at: 0 155 582
Size: 155 427
BtgI C'CryG_G
Cuts at: 0 46 202 582
Size: 46 156 380
Fragments arranged by size:
380 156 46
EaeI y'GGCC_r
Cuts at: 0 149 356 501 582
Size: 149 207 145 81
Fragments arranged by size:
207 149 145 81
EarI CTCTTCn'nnn_
Cuts at: 0 514 582
Size: 514 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 379 436 582
Size: 379 57 146
Fragments arranged by size:
379 146 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 150 319 581 582
Size: 106 44 169 262 1
Fragments arranged by size:
262 169 106 44 1
EcoO109I rG'GnC_Cy
Cuts at: 0 198 582
Size: 198 384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 369 582
Size: 369 213
HaeII r_GCGC'y
Cuts at: 0 399 582
Size: 399 183
MscI TGG'CCA
Cuts at: 0 151 358 582
Size: 151 207 224
Fragments arranged by size:
224 207 151
MslI CAynn'nnrTG
Cuts at: 0 253 582
Size: 253 329
MspA1I CmG'CkG
Cuts at: 0 48 582
Size: 48 534
PpuMI rG'GwC_Cy
Cuts at: 0 198 582
Size: 198 384
PshAI GACnn'nnGTC
Cuts at: 0 337 582
Size: 337 245
PstI C_TGCA'G
Cuts at: 0 332 582
Size: 332 250
SacII CC_GC'GG
Cuts at: 0 49 582
Size: 49 533
SfcI C'TryA_G
Cuts at: 0 328 582
Size: 328 254
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 509 582
Size: 509 73
SmaI CCC'GGG
Cuts at: 0 172 509 582
Size: 172 337 73
Fragments arranged by size:
337 172 73
SrfI GCCC'GGGC
Cuts at: 0 509 582
Size: 509 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 105 137 582
Size: 105 32 445
Fragments arranged by size:
445 105 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 550 582
Size: 550 32
XmaI C'CCGG_G
Cuts at: 0 170 507 582
Size: 170 337 75
Fragments arranged by size:
337 170 75
Enzymes that do cut:
AarI AcuI AlfI AloI ApoI AvaI BanII BbsI
BglI BpmI BsaI BsaHI BsaWI BseYI BsmI BsmBI
Bsp1286I BspEI BspMI BstBI BstYI BtgI EaeI EarI
EciI Eco57MI EcoO109I FalI HaeII MscI MslI MspA1I
PpuMI PshAI PstI SacII SfcI SfiI SmaI SrfI
TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaXI BseRI BsgI
BsiEI BsiHKAI BsiWI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI Tth111I XbaI XhoI XmnI
ZraI