(Linear) (Six-base) MAPSORT of: KIBB6855.seq  Check: 1127  from: 1  to: 534



KIBB6855.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     411     534 
   Size:        411     123
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     126     367     534 
   Size:        126     241     167

  Fragments arranged by size: 

                 241     167     126
 
BanI G'GyrC_C

Cuts at:      0      67     534 
   Size:         67     467
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     160     355     534 
   Size:        160     195     179

  Fragments arranged by size: 

                 195     179     160
 
BbvCI CC'TCA_GC

Cuts at:      0     361     534 
   Size:        361     173
 
BglII A'GATC_T

Cuts at:      0     126     534 
   Size:        126     408
 
BlpI GC'TnA_GC

Cuts at:      0       9     534 
   Size:          9     525
 
Bme1580I G_kGCm'C

Cuts at:      0      70     534 
   Size:         70     464
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      50      66     534 
   Size:         50      16     468

  Fragments arranged by size: 

                 468      50      16
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     292     534 
   Size:        232      60     242

  Fragments arranged by size: 

                 242     232      60
 
Bpu10I CC'TnA_GC

Cuts at:      0     361     534 
   Size:        361     173
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     289     534 
   Size:        289     245
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      72     534 
   Size:         72     462
 
BsmI GAATG_Cn'

Cuts at:      0     435     534 
   Size:        435      99
 
Bsp1286I G_dGCh'C

Cuts at:      0      70     534 
   Size:         70     464
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     116     411     534 
   Size:        116     295     123

  Fragments arranged by size: 

                 295     123     116
 
BstEII G'GTnAC_C

Cuts at:      0     226     534 
   Size:        226     308
 
BstYI r'GATC_y

Cuts at:      0     126     534 
   Size:        126     408
 
Bsu36I CC'TnA_GG

Cuts at:      0     355     534 
   Size:        355     179
 
BtgI C'CryG_G

Cuts at:      0     413     534 
   Size:        413     121
 
BtsI GCAGTG_nn'

Cuts at:      0      13     534 
   Size:         13     521
 
ClaI AT'CG_AT

Cuts at:      0     381     534 
   Size:        381     153
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     126     232     292     367     534 
   Size:        126     106      60      75     167

  Fragments arranged by size: 

                 167     126     106      75      60
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     274     534 
   Size:        274     260
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     177     222     450     534 
   Size:        177      45     228      84

  Fragments arranged by size: 

                 228     177      84      45
 
HaeII r_GCGC'y

Cuts at:      0     375     534 
   Size:        375     159
 
MslI CAynn'nnrTG

Cuts at:      0     517     534 
   Size:        517      17
 
MspA1I CmG'CkG

Cuts at:      0      86     180     534 
   Size:         86      94     354

  Fragments arranged by size: 

                 354      94      86
 
NspI r_CATG'y

Cuts at:      0     120     325     534 
   Size:        120     205     209

  Fragments arranged by size: 

                 209     205     120
 
PasI CC'CwG_GG

Cuts at:      0     333     534 
   Size:        333     201
 
PvuII CAG'CTG

Cuts at:      0      86     180     534 
   Size:         86      94     354

  Fragments arranged by size: 

                 354      94      86
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      85     106     534 
   Size:         85      21     428

  Fragments arranged by size: 

                 428      85      21
 
XmnI GAAnn'nnTTC

Cuts at:      0     157     534 
   Size:        157     377
 

 Enzymes that do cut:

     AarI     AcuI     BanI     BbsI    BbvCI    BglII     BlpI Bme1580I
     BmrI     BpmI   Bpu10I    BseRI     BsgI     BsmI Bsp1286I    BspMI
   BstEII    BstYI   Bsu36I     BtgI     BtsI     ClaI  Eco57MI    EcoNI
     FalI    HaeII     MslI   MspA1I     NspI     PasI    PvuII    TaqII
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
  BstZ17I    BtgZI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI