(Linear) (Six-base) MAPSORT of: KIBB6856.seq  Check: 2120  from: 1  to: 582



KIBB6856.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     241     273     582 
   Size:        241      32     309

  Fragments arranged by size: 

                 309     241      32
 
ApaLI G'TGCA_C

Cuts at:      0     373     582 
   Size:        373     209
 
ApoI r'AATT_y

Cuts at:      0     296     562     582 
   Size:        296     266      20

  Fragments arranged by size: 

                 296     266      20
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     393     582 
   Size:        393     189
 
BlpI GC'TnA_GC

Cuts at:      0     113     582 
   Size:        113     469
 
Bme1580I G_kGCm'C

Cuts at:      0     377     582 
   Size:        377     205
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     114     377     582 
   Size:        114     263     205

  Fragments arranged by size: 

                 263     205     114
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     109     582 
   Size:        103       6     473

  Fragments arranged by size: 

                 473     103       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     118     143     305     582 
   Size:        118      25     162     277

  Fragments arranged by size: 

                 277     162     118      25
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     409     582 
   Size:         91     318     173

  Fragments arranged by size: 

                 318     173      91
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     241     271     582 
   Size:        241      30     311

  Fragments arranged by size: 

                 311     241      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     306     582 
   Size:        306     276
 
BsiHKAI G_wGCw'C

Cuts at:      0     377     582 
   Size:        377     205
 
Bsp1286I G_dGCh'C

Cuts at:      0     377     582 
   Size:        377     205
 
BsrFI r'CCGG_y

Cuts at:      0     452     582 
   Size:        452     130
 
EarI CTCTTCn'nnn_

Cuts at:      0      83     582 
   Size:         83     499
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     109     582 
   Size:        103       6     473

  Fragments arranged by size: 

                 473     103       6
 
EcoRI G'AATT_C

Cuts at:      0     296     562     582 
   Size:        296     266      20

  Fragments arranged by size: 

                 296     266      20
 
HaeII r_GCGC'y

Cuts at:      0      17     582 
   Size:         17     565
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      43      75     582 
   Size:         43      32     507

  Fragments arranged by size: 

                 507      43      32
 
MfeI C'AATT_G

Cuts at:      0     577     582 
   Size:        577       5
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     582 
   Size:        148     434
 
MspA1I CmG'CkG

Cuts at:      0     370     582 
   Size:        370     212
 
NaeI GCC'GGC

Cuts at:      0     454     582 
   Size:        454     128
 
NgoMIV G'CCGG_C

Cuts at:      0     452     582 
   Size:        452     130
 
NspI r_CATG'y

Cuts at:      0     274     582 
   Size:        274     308
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     241     273     582 
   Size:        241      32     309

  Fragments arranged by size: 

                 309     241      32
 
PvuII CAG'CTG

Cuts at:      0     370     582 
   Size:        370     212
 
SapI GCTCTTCn'nnn_

Cuts at:      0      83     582 
   Size:         83     499
 
SmlI C'TyrA_G

Cuts at:      0     106     388     582 
   Size:        106     282     194

  Fragments arranged by size: 

                 282     194     106
 
SspI AAT'ATT

Cuts at:      0     132     582 
   Size:        132     450
 
Tth111I GACn'n_nGTC

Cuts at:      0     397     582 
   Size:        397     185
 
XmnI GAAnn'nnTTC

Cuts at:      0     211     256     582 
   Size:        211      45     326

  Fragments arranged by size: 

                 326     211      45
 

 Enzymes that do cut:

     AloI    ApaLI     ApoI     BbsI     BlpI Bme1580I     BplI     BpmI
   Bpu10I    BpuEI    BsaXI    BseRI  BsiHKAI Bsp1286I    BsrFI     EarI
  Eco57MI    EcoRI    HaeII    Hin4I     MfeI     MmeI   MspA1I     NaeI
   NgoMIV     NspI     PpiI    PvuII     SapI     SmlI     SspI  Tth111I
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmrI     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MscI     MslI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TatI     TstI     XbaI     XcmI
     XhoI     XmaI     ZraI