(Linear) (Six-base) MAPSORT of: KIBB6858.seq  Check: 9437  from: 1  to: 582



KIBB6858.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     582 
   Size:         51     531
 
ApoI r'AATT_y

Cuts at:      0     478     582 
   Size:        478     104
 
AvaI C'yCGr_G

Cuts at:      0      19     582 
   Size:         19     563
 
BanII G_rGCy'C

Cuts at:      0      49     184     582 
   Size:         49     135     398

  Fragments arranged by size: 

                 398     135      49
 
BbvCI CC'TCA_GC

Cuts at:      0     427     582 
   Size:        427     155
 
BglI GCCn_nnn'nGGC

Cuts at:      0      44     582 
   Size:         44     538
 
Bpu10I CC'TnA_GC

Cuts at:      0     427     582 
   Size:        427     155
 
BsaHI Gr'CG_yC

Cuts at:      0     555     582 
   Size:        555      27
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46     582 
   Size:         46     536
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     582 
   Size:         46     536
 
BsiEI CG_ry'CG

Cuts at:      0     370     582 
   Size:        370     212
 
BsiHKAI G_wGCw'C

Cuts at:      0     184     582 
   Size:        184     398
 
Bsp1286I G_dGCh'C

Cuts at:      0      49     184     582 
   Size:         49     135     398

  Fragments arranged by size: 

                 398     135      49
 
BspHI T'CATG_A

Cuts at:      0     449     582 
   Size:        449     133
 
BssSI C'ACGA_G

Cuts at:      0     441     582 
   Size:        441     141
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     259     534     582 
   Size:        259     275      48

  Fragments arranged by size: 

                 275     259      48
 
BstYI r'GATC_y

Cuts at:      0     539     582 
   Size:        539      43
 
DraI TTT'AAA

Cuts at:      0     402     582 
   Size:        402     180
 
EaeI y'GGCC_r

Cuts at:      0     367     419     524     582 
   Size:        367      52     105      58

  Fragments arranged by size: 

                 367     105      58      52
 
EagI C'GGCC_G

Cuts at:      0     367     582 
   Size:        367     215
 
EcoICRI GAG'CTC

Cuts at:      0     182     582 
   Size:        182     400
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     582 
   Size:         51     531
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     511     582 
   Size:        511      71
 
HaeII r_GCGC'y

Cuts at:      0      19     582 
   Size:         19     563
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     524     556     582 
   Size:        524      32      26

  Fragments arranged by size: 

                 524      32      26
 
MscI TGG'CCA

Cuts at:      0     526     582 
   Size:        526      56
 
PsiI TTA'TAA

Cuts at:      0     116     582 
   Size:        116     466
 
PstI C_TGCA'G

Cuts at:      0      44     477     582 
   Size:         44     433     105

  Fragments arranged by size: 

                 433     105      44
 
SacI G_AGCT'C

Cuts at:      0     184     582 
   Size:        184     398
 
SbfI CC_TGCA'GG

Cuts at:      0      44     582 
   Size:         44     538
 
SfcI C'TryA_G

Cuts at:      0      40     473     582 
   Size:         40     433     109

  Fragments arranged by size: 

                 433     109      40
 
SmaI CCC'GGG

Cuts at:      0      21     582 
   Size:         21     561
 
SspI AAT'ATT

Cuts at:      0     378     582 
   Size:        378     204
 
StyI C'CwwG_G

Cuts at:      0     386     582 
   Size:        386     196
 
XmaI C'CCGG_G

Cuts at:      0      19     582 
   Size:         19     563
 
XmnI GAAnn'nnTTC

Cuts at:      0     377     490     582 
   Size:        377     113      92

  Fragments arranged by size: 

                 377     113      92
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI    BanII    BbvCI     BglI   Bpu10I    BsaHI
    BseRI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BssSI    BstXI
    BstYI     DraI     EaeI     EagI  EcoICRI  Eco57MI     FalI    HaeII
    Hin4I     MscI     PsiI     PstI     SacI     SbfI     SfcI     SmaI
     SspI     StyI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseYI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI