(Linear) (Six-base) MAPSORT of: KIBB6859.seq  Check: 5634  from: 1  to: 537



KIBB6859.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     154     537 
   Size:        154     383
 
AvrII C'CTAG_G

Cuts at:      0     474     537 
   Size:        474      63
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      35     246     537 
   Size:         35     211     291

  Fragments arranged by size: 

                 291     211      35
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     537 
   Size:        111     426
 
BseYI C'CCAG_C

Cuts at:      0     405     537 
   Size:        405     132
 
BsiHKAI G_wGCw'C

Cuts at:      0     259     537 
   Size:        259     278
 
Bsp1286I G_dGCh'C

Cuts at:      0     259     537 
   Size:        259     278
 
BstBI TT'CG_AA

Cuts at:      0     213     537 
   Size:        213     324
 
BstEII G'GTnAC_C

Cuts at:      0     520     537 
   Size:        520      17
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      15     348     537 
   Size:         15     333     189

  Fragments arranged by size: 

                 333     189      15
 
EaeI y'GGCC_r

Cuts at:      0     333     537 
   Size:        333     204
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     537 
   Size:        111     426
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     537 
   Size:         42     495
 
MscI TGG'CCA

Cuts at:      0     335     537 
   Size:        335     202
 
MspA1I CmG'CkG

Cuts at:      0     185     537 
   Size:        185     352
 
NspI r_CATG'y

Cuts at:      0      81     537 
   Size:         81     456
 
SspI AAT'ATT

Cuts at:      0      28     537 
   Size:         28     509
 
StyI C'CwwG_G

Cuts at:      0      14     341     474     537 
   Size:         14     327     133      63

  Fragments arranged by size: 

                 327     133      63      14
 
TatI w'GTAC_w

Cuts at:      0      43     191     537 
   Size:         43     148     346

  Fragments arranged by size: 

                 346     148      43
 
Tth111I GACn'n_nGTC

Cuts at:      0     511     537 
   Size:        511      26
 

 Enzymes that do cut:

    AlwNI    AvrII     BmrI     BpmI    BseYI  BsiHKAI Bsp1286I    BstBI
   BstEII    BstXI     EaeI  Eco57MI     MmeI     MscI   MspA1I     NspI
     SspI     StyI     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI