Restriction map for
pF1KB6860
(Linear) (Six-base) MAPSORT of: KIBB6860.seq Check: 6473 from: 1 to: 561
KIBB6860.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 561
Size: 405 156
AfeI AGC'GCT
Cuts at: 0 178 561
Size: 178 383
AleI CACnn'nnGTG
Cuts at: 0 202 561
Size: 202 359
AlwNI CAG_nnn'CTG
Cuts at: 0 261 561
Size: 261 300
ApaI G_GGCC'C
Cuts at: 0 57 64 98 477 561
Size: 57 7 34 379 84
Fragments arranged by size:
379 84 57 34 7
AvaI C'yCGr_G
Cuts at: 0 57 63 87 107 561
Size: 57 6 24 20 454
Fragments arranged by size:
454 57 24 20 6
BanI G'GyrC_C
Cuts at: 0 225 561
Size: 225 336
BanII G_rGCy'C
Cuts at: 0 57 64 98 307 477 561
Size: 57 7 34 209 170 84
Fragments arranged by size:
209 170 84 57 34 7
BbvCI CC'TCA_GC
Cuts at: 0 389 561
Size: 389 172
BglI GCCn_nnn'nGGC
Cuts at: 0 361 561
Size: 361 200
Bme1580I G_kGCm'C
Cuts at: 0 57 64 98 477 561
Size: 57 7 34 379 84
Fragments arranged by size:
379 84 57 34 7
BmgBI CAC'GTC
Cuts at: 0 168 561
Size: 168 393
Bpu10I CC'TnA_GC
Cuts at: 0 389 561
Size: 389 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 561
Size: 342 219
BseYI C'CCAG_C
Cuts at: 0 476 521 555 561
Size: 476 45 34 6
Fragments arranged by size:
476 45 34 6
BsiHKAI G_wGCw'C
Cuts at: 0 251 307 561
Size: 251 56 254
Fragments arranged by size:
254 251 56
Bsp1286I G_dGCh'C
Cuts at: 0 57 64 98 251 307 477 561
Size: 57 7 34 153 56 170 84
Fragments arranged by size:
170 153 84 57 56 34 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 269 561
Size: 269 292
BsrBI CCG'CTC
Cuts at: 0 114 561
Size: 114 447
BstYI r'GATC_y
Cuts at: 0 485 561
Size: 485 76
BtgI C'CryG_G
Cuts at: 0 49 81 323 464 561
Size: 49 32 242 141 97
Fragments arranged by size:
242 141 97 49 32
BtsI GCAGTG_nn'
Cuts at: 0 513 561
Size: 513 48
DrdI GACnn_nn'nnGTC
Cuts at: 0 424 561
Size: 424 137
EaeI y'GGCC_r
Cuts at: 0 320 561
Size: 320 241
EarI CTCTTCn'nnn_
Cuts at: 0 16 561
Size: 16 545
EcoICRI GAG'CTC
Cuts at: 0 305 561
Size: 305 256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 561
Size: 405 156
EcoO109I rG'GnC_Cy
Cuts at: 0 54 94 95 258 473 561
Size: 54 40 1 163 215 88
Fragments arranged by size:
215 163 88 54 40 1
HaeII r_GCGC'y
Cuts at: 0 21 180 561
Size: 21 159 381
Fragments arranged by size:
381 159 21
MscI TGG'CCA
Cuts at: 0 322 561
Size: 322 239
MslI CAynn'nnrTG
Cuts at: 0 202 561
Size: 202 359
MspA1I CmG'CkG
Cuts at: 0 51 480 492 561
Size: 51 429 12 69
Fragments arranged by size:
429 69 51 12
NcoI C'CATG_G
Cuts at: 0 323 561
Size: 323 238
PflMI CCAn_nnn'nTGG
Cuts at: 0 329 561
Size: 329 232
PfoI T'CCnGG_A
Cuts at: 0 100 561
Size: 100 461
PpuMI rG'GwC_Cy
Cuts at: 0 258 561
Size: 258 303
PspOMI G'GGCC_C
Cuts at: 0 53 60 94 473 561
Size: 53 7 34 379 88
Fragments arranged by size:
379 88 53 34 7
PstI C_TGCA'G
Cuts at: 0 224 561
Size: 224 337
PvuII CAG'CTG
Cuts at: 0 492 561
Size: 492 69
SacI G_AGCT'C
Cuts at: 0 307 561
Size: 307 254
SacII CC_GC'GG
Cuts at: 0 52 561
Size: 52 509
SfcI C'TryA_G
Cuts at: 0 220 561
Size: 220 341
SmaI CCC'GGG
Cuts at: 0 59 109 561
Size: 59 50 452
Fragments arranged by size:
452 59 50
SmlI C'TyrA_G
Cuts at: 0 357 561
Size: 357 204
StyI C'CwwG_G
Cuts at: 0 323 561
Size: 323 238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 462 561
Size: 462 99
XmaI C'CCGG_G
Cuts at: 0 57 107 561
Size: 57 50 454
Fragments arranged by size:
454 57 50
XmnI GAAnn'nnTTC
Cuts at: 0 274 561
Size: 274 287
Enzymes that do cut:
AcuI AfeI AleI AlwNI ApaI AvaI BanI BanII
BbvCI BglI Bme1580I BmgBI Bpu10I BpuEI BseYI BsiHKAI
Bsp1286I BspMI BsrBI BstYI BtgI BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I HaeII MscI MslI MspA1I
NcoI PflMI PfoI PpuMI PspOMI PstI PvuII SacI
SacII SfcI SmaI SmlI StyI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI ZraI