Restriction map for
pF1KB6862
(Linear) (Six-base) MAPSORT of: KIBB6862.seq Check: 7487 from: 1 to: 585
KIBB6862.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AclI AA'CG_TT
Cuts at: 0 383 585
Size: 383 202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 471 585
Size: 94 377 114
Fragments arranged by size:
377 114 94
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 374 541 585
Size: 374 167 44
Fragments arranged by size:
374 167 44
BamHI G'GATC_C
Cuts at: 0 296 585
Size: 296 289
BanI G'GyrC_C
Cuts at: 0 87 193 585
Size: 87 106 392
Fragments arranged by size:
392 106 87
BanII G_rGCy'C
Cuts at: 0 59 203 277 458 585
Size: 59 144 74 181 127
Fragments arranged by size:
181 144 127 74 59
BbeI G_GCGC'C
Cuts at: 0 197 585
Size: 197 388
BmrI ACTGGGnnnn_n'
Cuts at: 0 546 585
Size: 546 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 202 299 517 585
Size: 55 147 97 218 68
Fragments arranged by size:
218 147 97 68 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 585
Size: 43 542
BsaI GGTCTCn'nnnn_
Cuts at: 0 178 585
Size: 178 407
BsaHI Gr'CG_yC
Cuts at: 0 17 143 194 585
Size: 17 126 51 391
Fragments arranged by size:
391 126 51 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 585
Size: 538 30 17
Fragments arranged by size:
538 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 299 520 585
Size: 67 232 221 65
Fragments arranged by size:
232 221 67 65
BseYI C'CCAG_C
Cuts at: 0 292 585
Size: 292 293
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 585
Size: 319 266
BsiHKAI G_wGCw'C
Cuts at: 0 59 203 585
Size: 59 144 382
Fragments arranged by size:
382 144 59
Bsp1286I G_dGCh'C
Cuts at: 0 59 203 277 458 585
Size: 59 144 74 181 127
Fragments arranged by size:
181 144 127 74 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 523 585
Size: 523 62
BsrBI CCG'CTC
Cuts at: 0 579 585
Size: 579 6
BssHII G'CGCG_C
Cuts at: 0 6 8 10 125 585
Size: 6 2 2 115 460
Fragments arranged by size:
460 115 6 2 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 277 585
Size: 277 308
BstYI r'GATC_y
Cuts at: 0 296 585
Size: 296 289
Bsu36I CC'TnA_GG
Cuts at: 0 541 585
Size: 541 44
BtgI C'CryG_G
Cuts at: 0 118 137 585
Size: 118 19 448
Fragments arranged by size:
448 118 19
BtsI GCAGTG_nn'
Cuts at: 0 449 585
Size: 449 136
DrdI GACnn_nn'nnGTC
Cuts at: 0 166 585
Size: 166 419
EaeI y'GGCC_r
Cuts at: 0 242 260 585
Size: 242 18 325
Fragments arranged by size:
325 242 18
EarI CTCTTCn'nnn_
Cuts at: 0 490 585
Size: 490 95
EcoICRI GAG'CTC
Cuts at: 0 57 201 585
Size: 57 144 384
Fragments arranged by size:
384 144 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 94 202 299 471 517 585
Size: 55 39 108 97 172 46 68
Fragments arranged by size:
172 108 97 68 55 46 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 565 585
Size: 565 20
EcoO109I rG'GnC_Cy
Cuts at: 0 303 417 537 570 585
Size: 303 114 120 33 15
Fragments arranged by size:
303 120 114 33 15
HaeII r_GCGC'y
Cuts at: 0 197 585
Size: 197 388
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 585
Size: 538 32 15
Fragments arranged by size:
538 32 15
KasI G'GCGC_C
Cuts at: 0 193 585
Size: 193 392
MspA1I CmG'CkG
Cuts at: 0 93 120 252 292 585
Size: 93 27 132 40 293
Fragments arranged by size:
293 132 93 40 27
NarI GG'CG_CC
Cuts at: 0 194 585
Size: 194 391
NcoI C'CATG_G
Cuts at: 0 137 585
Size: 137 448
NruI TCG'CGA
Cuts at: 0 370 585
Size: 370 215
PfoI T'CCnGG_A
Cuts at: 0 23 585
Size: 23 562
PpuMI rG'GwC_Cy
Cuts at: 0 417 570 585
Size: 417 153 15
Fragments arranged by size:
417 153 15
PstI C_TGCA'G
Cuts at: 0 236 257 585
Size: 236 21 328
Fragments arranged by size:
328 236 21
PvuII CAG'CTG
Cuts at: 0 252 292 585
Size: 252 40 293
Fragments arranged by size:
293 252 40
SacI G_AGCT'C
Cuts at: 0 59 203 585
Size: 59 144 382
Fragments arranged by size:
382 144 59
SacII CC_GC'GG
Cuts at: 0 121 585
Size: 121 464
SapI GCTCTTCn'nnn_
Cuts at: 0 490 585
Size: 490 95
SbfI CC_TGCA'GG
Cuts at: 0 236 585
Size: 236 349
SexAI A'CCwGG_T
Cuts at: 0 560 585
Size: 560 25
SfcI C'TryA_G
Cuts at: 0 232 253 585
Size: 232 21 332
Fragments arranged by size:
332 232 21
SfoI GGC'GCC
Cuts at: 0 195 585
Size: 195 390
SmlI C'TyrA_G
Cuts at: 0 58 585
Size: 58 527
StuI AGG'CCT
Cuts at: 0 332 585
Size: 332 253
StyI C'CwwG_G
Cuts at: 0 137 146 585
Size: 137 9 439
Fragments arranged by size:
439 137 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 506 585
Size: 144 362 79
Fragments arranged by size:
362 144 79
XmnI GAAnn'nnTTC
Cuts at: 0 484 585
Size: 484 101
Enzymes that do cut:
AclI AcuI AlfI BamHI BanI BanII BbeI BmrI
BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BssHII BstXI BstYI Bsu36I
BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MspA1I NarI NcoI NruI
PfoI PpuMI PstI PvuII SacI SacII SapI SbfI
SexAI SfcI SfoI SmlI StuI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NaeI NdeI NgoMIV NheI NotI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI
SanDI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI ZraI