(Linear) (Six-base) MAPSORT of: KIBB6863.seq  Check: 5888  from: 1  to: 537



KIBB6863.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AflII C'TTAA_G

Cuts at:      0     526     537 
   Size:        526      11
 
AflIII A'CryG_T

Cuts at:      0     178     537 
   Size:        178     359
 
ApoI r'AATT_y

Cuts at:      0       5     537 
   Size:          5     532
 
AseI AT'TA_AT

Cuts at:      0     170     537 
   Size:        170     367
 
BfrBI ATG'CAT

Cuts at:      0     395     537 
   Size:        395     142
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     411     537 
   Size:        411     126
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     331     537 
   Size:        331     206
 
BsiHKAI G_wGCw'C

Cuts at:      0      50     477     537 
   Size:         50     427      60

  Fragments arranged by size: 

                 427      60      50
 
BsmI GAATG_Cn'

Cuts at:      0     397     537 
   Size:        397     140
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     477     537 
   Size:         50     427      60

  Fragments arranged by size: 

                 427      60      50
 
BsrGI T'GTAC_A

Cuts at:      0     123     537 
   Size:        123     414
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     472     537 
   Size:        472      65
 
EaeI y'GGCC_r

Cuts at:      0     204     537 
   Size:        204     333
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     152     221     537 
   Size:         16     136      69     316

  Fragments arranged by size: 

                 316     136      69      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     140     537 
   Size:        140     397
 
EcoO109I rG'GnC_Cy

Cuts at:      0     339     537 
   Size:        339     198
 
EcoRI G'AATT_C

Cuts at:      0       5     537 
   Size:          5     532
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     232     537 
   Size:        232     305
 
MscI TGG'CCA

Cuts at:      0     206     537 
   Size:        206     331
 
NsiI A_TGCA'T

Cuts at:      0     397     537 
   Size:        397     140
 
NspI r_CATG'y

Cuts at:      0     470     537 
   Size:        470      67
 
PfoI T'CCnGG_A

Cuts at:      0     226     537 
   Size:        226     311
 
PpuMI rG'GwC_Cy

Cuts at:      0     339     537 
   Size:        339     198
 
SfcI C'TryA_G

Cuts at:      0     401     537 
   Size:        401     136
 
SmlI C'TyrA_G

Cuts at:      0     526     537 
   Size:        526      11
 
SphI G_CATG'C

Cuts at:      0     470     537 
   Size:        470      67
 
StyI C'CwwG_G

Cuts at:      0     498     537 
   Size:        498      39
 
TatI w'GTAC_w

Cuts at:      0     123     277     537 
   Size:        123     154     260

  Fragments arranged by size: 

                 260     154     123
 
XmnI GAAnn'nnTTC

Cuts at:      0     215     537 
   Size:        215     322
 

 Enzymes that do cut:

    AflII   AflIII     ApoI     AseI    BfrBI     BmrI     BplI  BsiHKAI
     BsmI Bsp1286I    BsrGI   BstAPI     EaeI     EarI    EcoNI EcoO109I
    EcoRI     FalI     MscI     NsiI     NspI     PfoI    PpuMI     SfcI
     SmlI     SphI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI  Eco57MI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI