Restriction map for
pF1KB6866
(Linear) (Six-base) MAPSORT of: KIBB6866.seq Check: 8944 from: 1 to: 588
KIBB6866.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 133 588
Size: 133 455
ApaI G_GGCC'C
Cuts at: 0 490 537 588
Size: 490 47 51
Fragments arranged by size:
490 51 47
AvaI C'yCGr_G
Cuts at: 0 483 490 536 588
Size: 483 7 46 52
Fragments arranged by size:
483 52 46 7
BamHI G'GATC_C
Cuts at: 0 330 413 588
Size: 330 83 175
Fragments arranged by size:
330 175 83
BanII G_rGCy'C
Cuts at: 0 490 503 537 588
Size: 490 13 34 51
Fragments arranged by size:
490 51 34 13
Bme1580I G_kGCm'C
Cuts at: 0 490 522 537 588
Size: 490 32 15 51
Fragments arranged by size:
490 51 32 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 106 186 588
Size: 106 80 402
Fragments arranged by size:
402 106 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 588
Size: 260 328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 588
Size: 290 298
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 233 263 358 388 588
Size: 233 30 95 30 200
Fragments arranged by size:
233 200 95 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 379 392 588
Size: 379 13 196
Fragments arranged by size:
379 196 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 164 588
Size: 63 101 424
Fragments arranged by size:
424 101 63
BsiHKAI G_wGCw'C
Cuts at: 0 503 588
Size: 503 85
Bsp1286I G_dGCh'C
Cuts at: 0 490 503 522 537 588
Size: 490 13 19 15 51
Fragments arranged by size:
490 51 19 15 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 284 588
Size: 284 304
BstYI r'GATC_y
Cuts at: 0 84 330 413 588
Size: 84 246 83 175
Fragments arranged by size:
246 175 84 83
Bsu36I CC'TnA_GG
Cuts at: 0 404 588
Size: 404 184
BtsI GCAGTG_nn'
Cuts at: 0 35 588
Size: 35 553
EarI CTCTTCn'nnn_
Cuts at: 0 248 588
Size: 248 340
EcoICRI GAG'CTC
Cuts at: 0 501 588
Size: 501 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 588
Size: 260 328
EcoO109I rG'GnC_Cy
Cuts at: 0 135 258 346 487 533 588
Size: 135 123 88 141 46 55
Fragments arranged by size:
141 135 123 88 55 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 119 151 405 437 457 489 588
Size: 119 32 254 32 20 32 99
Fragments arranged by size:
254 119 99 32 32 32 20
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 391 588
Size: 358 33 197
Fragments arranged by size:
358 197 33
MspA1I CmG'CkG
Cuts at: 0 264 393 588
Size: 264 129 195
Fragments arranged by size:
264 195 129
PflMI CCAn_nnn'nTGG
Cuts at: 0 340 588
Size: 340 248
PfoI T'CCnGG_A
Cuts at: 0 423 548 588
Size: 423 125 40
Fragments arranged by size:
423 125 40
PpuMI rG'GwC_Cy
Cuts at: 0 135 258 588
Size: 135 123 330
Fragments arranged by size:
330 135 123
PshAI GACnn'nnGTC
Cuts at: 0 300 588
Size: 300 288
PspOMI G'GGCC_C
Cuts at: 0 486 533 588
Size: 486 47 55
Fragments arranged by size:
486 55 47
PstI C_TGCA'G
Cuts at: 0 57 363 392 582 588
Size: 57 306 29 190 6
Fragments arranged by size:
306 190 57 29 6
PvuII CAG'CTG
Cuts at: 0 393 588
Size: 393 195
SacI G_AGCT'C
Cuts at: 0 503 588
Size: 503 85
ScaI AGT'ACT
Cuts at: 0 442 588
Size: 442 146
SfcI C'TryA_G
Cuts at: 0 53 286 359 388 578 588
Size: 53 233 73 29 190 10
Fragments arranged by size:
233 190 73 53 29 10
SmaI CCC'GGG
Cuts at: 0 538 588
Size: 538 50
SmlI C'TyrA_G
Cuts at: 0 305 588
Size: 305 283
SrfI GCCC'GGGC
Cuts at: 0 538 588
Size: 538 50
StuI AGG'CCT
Cuts at: 0 121 481 559 588
Size: 121 360 78 29
Fragments arranged by size:
360 121 78 29
TatI w'GTAC_w
Cuts at: 0 440 588
Size: 440 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 86 118 233 265 358 390 588
Size: 86 32 115 32 93 32 198
Fragments arranged by size:
198 115 93 86 32 32 32
XmaI C'CCGG_G
Cuts at: 0 536 588
Size: 536 52
Enzymes that do cut:
AhdI ApaI AvaI BamHI BanII Bme1580I BmrI BpmI
BpuEI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI BstYI
Bsu36I BtsI EarI EcoICRI Eco57MI EcoO109I Hin4I MmeI
MspA1I PflMI PfoI PpuMI PshAI PspOMI PstI PvuII
SacI ScaI SfcI SmaI SmlI SrfI StuI TatI
TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SspI StyI SwaI TaqII Tth111I XbaI XcmI XhoI
XmnI ZraI