(Linear) (Six-base) MAPSORT of: KIBB6871.seq  Check: 9497  from: 1  to: 540



KIBB6871.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     540 
   Size:        130     410
 
AhdI GACnn_n'nnGTC

Cuts at:      0     516     540 
   Size:        516      24
 
BglI GCCn_nnn'nGGC

Cuts at:      0     231     540 
   Size:        231     309
 
BmgBI CAC'GTC

Cuts at:      0     201     540 
   Size:        201     339
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     540 
   Size:        304     236
 
BsaAI yAC'GTr

Cuts at:      0     455     540 
   Size:        455      85
 
BseYI C'CCAG_C

Cuts at:      0     426     540 
   Size:        426     114
 
BsrBI CCG'CTC

Cuts at:      0     294     540 
   Size:        294     246
 
BsrGI T'GTAC_A

Cuts at:      0     101     540 
   Size:        101     439
 
BssSI C'ACGA_G

Cuts at:      0     523     540 
   Size:        523      17
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     387     540 
   Size:        387     153
 
BstYI r'GATC_y

Cuts at:      0      32     540 
   Size:         32     508
 
BtsI GCAGTG_nn'

Cuts at:      0     362     540 
   Size:        362     178
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     304     540 
   Size:        130     174     236

  Fragments arranged by size: 

                 236     174     130
 
EcoO109I rG'GnC_Cy

Cuts at:      0     258     540 
   Size:        258     282
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     210     242     502     534     540 
   Size:        210      32     260      32       6

  Fragments arranged by size: 

                 260     210      32      32       6
 
MslI CAynn'nnrTG

Cuts at:      0     268     403     540 
   Size:        268     135     137

  Fragments arranged by size: 

                 268     137     135
 
NspI r_CATG'y

Cuts at:      0     390     540 
   Size:        390     150
 
PmlI CAC'GTG

Cuts at:      0     455     540 
   Size:        455      85
 
PstI C_TGCA'G

Cuts at:      0     335     540 
   Size:        335     205
 
SfcI C'TryA_G

Cuts at:      0     331     433     540 
   Size:        331     102     107

  Fragments arranged by size: 

                 331     107     102
 
StyI C'CwwG_G

Cuts at:      0     483     540 
   Size:        483      57
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     496     540 
   Size:        496      44
 
TatI w'GTAC_w

Cuts at:      0     101     540 
   Size:        101     439
 

 Enzymes that do cut:

     AcuI     AhdI     BglI    BmgBI     BpmI    BsaAI    BseYI    BsrBI
    BsrGI    BssSI   BstAPI    BstYI     BtsI  Eco57MI EcoO109I    Hin4I
     MslI     NspI     PmlI     PstI     SfcI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI