(Linear) (Six-base) MAPSORT of: KIBB6872.seq  Check: 3368  from: 1  to: 594



KIBB6872.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18     514     594 
   Size:         18     496      80

  Fragments arranged by size: 

                 496      80      18
 
AflIII A'CryG_T

Cuts at:      0     156     594 
   Size:        156     438
 
AseI AT'TA_AT

Cuts at:      0     278     594 
   Size:        278     316
 
AvrII C'CTAG_G

Cuts at:      0      54     594 
   Size:         54     540
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     229     263     594 
   Size:        229      34     331

  Fragments arranged by size: 

                 331     229      34
 
Bme1580I G_kGCm'C

Cuts at:      0     236     594 
   Size:        236     358
 
BmgBI CAC'GTC

Cuts at:      0     247     594 
   Size:        247     347
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     576     594 
   Size:        576      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     594 
   Size:         31     563
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     526     594 
   Size:        526      68
 
BseYI C'CCAG_C

Cuts at:      0     270     594 
   Size:        270     324
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     552     594 
   Size:        326     226      42

  Fragments arranged by size: 

                 326     226      42
 
BsiHKAI G_wGCw'C

Cuts at:      0      47     542     594 
   Size:         47     495      52

  Fragments arranged by size: 

                 495      52      47
 
BsmI GAATG_Cn'

Cuts at:      0     510     594 
   Size:        510      84
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     236     542     594 
   Size:         47     189     306      52

  Fragments arranged by size: 

                 306     189      52      47
 
BsrGI T'GTAC_A

Cuts at:      0      62     594 
   Size:         62     532
 
BssSI C'ACGA_G

Cuts at:      0     235     322     594 
   Size:        235      87     272

  Fragments arranged by size: 

                 272     235      87
 
BstYI r'GATC_y

Cuts at:      0      20     594 
   Size:         20     574
 
BtgI C'CryG_G

Cuts at:      0     515     594 
   Size:        515      79
 
BtsI GCAGTG_nn'

Cuts at:      0      45     540     594 
   Size:         45     495      54

  Fragments arranged by size: 

                 495      54      45
 
EarI CTCTTCn'nnn_

Cuts at:      0     125     179     594 
   Size:        125      54     415

  Fragments arranged by size: 

                 415     125      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18      31     514     594 
   Size:         18      13     483      80

  Fragments arranged by size: 

                 483      80      18      13
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     358     594 
   Size:        358     236
 
MfeI C'AATT_G

Cuts at:      0     194     594 
   Size:        194     400
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     199     594 
   Size:        187      12     395

  Fragments arranged by size: 

                 395     187      12
 
MslI CAynn'nnrTG

Cuts at:      0     520     594 
   Size:        520      74
 
MspA1I CmG'CkG

Cuts at:      0     572     594 
   Size:        572      22
 
NcoI C'CATG_G

Cuts at:      0     515     594 
   Size:        515      79
 
NspI r_CATG'y

Cuts at:      0     132     160     594 
   Size:        132      28     434

  Fragments arranged by size: 

                 434     132      28
 
PciI A'CATG_T

Cuts at:      0     156     594 
   Size:        156     438
 
SapI GCTCTTCn'nnn_

Cuts at:      0     179     594 
   Size:        179     415
 
SmlI C'TyrA_G

Cuts at:      0     541     594 
   Size:        541      53
 
StyI C'CwwG_G

Cuts at:      0      54     515     594 
   Size:         54     461      79

  Fragments arranged by size: 

                 461      79      54
 
TatI w'GTAC_w

Cuts at:      0      62     594 
   Size:         62     532
 

 Enzymes that do cut:

     AcuI   AflIII     AseI    AvrII     BcgI Bme1580I    BmgBI     BmrI
     BpmI    BpuEI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrGI
    BssSI    BstYI     BtgI     BtsI     EarI  Eco57MI     FalI     MfeI
     MmeI     MslI   MspA1I     NcoI     NspI     PciI     SapI     SmlI
     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI