(Linear) (Six-base) MAPSORT of: KIBB6874.seq  Check: 3  from: 1  to: 594



KIBB6874.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248     594 
   Size:        248     346
 
AlwNI CAG_nnn'CTG

Cuts at:      0     283     594 
   Size:        283     311
 
ApaI G_GGCC'C

Cuts at:      0     152     594 
   Size:        152     442
 
AvaI C'yCGr_G

Cuts at:      0     151     594 
   Size:        151     443
 
BanI G'GyrC_C

Cuts at:      0     246     594 
   Size:        246     348
 
BanII G_rGCy'C

Cuts at:      0     123     152     594 
   Size:        123      29     442

  Fragments arranged by size: 

                 442     123      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     215     477     594 
   Size:        215     262     117

  Fragments arranged by size: 

                 262     215     117
 
BglII A'GATC_T

Cuts at:      0     192     594 
   Size:        192     402
 
Bme1580I G_kGCm'C

Cuts at:      0     152     594 
   Size:        152     442
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     594 
   Size:        209     385
 
BsaWI w'CCGG_w

Cuts at:      0     115     594 
   Size:        115     479
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     334     594 
   Size:        334     260
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     594 
   Size:        424     170
 
Bsp1286I G_dGCh'C

Cuts at:      0     123     152     594 
   Size:        123      29     442

  Fragments arranged by size: 

                 442     123      29
 
BspEI T'CCGG_A

Cuts at:      0     115     594 
   Size:        115     479
 
BstYI r'GATC_y

Cuts at:      0     192     594 
   Size:        192     402
 
EarI CTCTTCn'nnn_

Cuts at:      0      40     353     594 
   Size:         40     313     241

  Fragments arranged by size: 

                 313     241      40
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      59     594 
   Size:         59     535
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     248     594 
   Size:        209      39     346

  Fragments arranged by size: 

                 346     209      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0      90     148     435     594 
   Size:         90      58     287     159

  Fragments arranged by size: 

                 287     159      90      58
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     358     390     594 
   Size:        358      32     204

  Fragments arranged by size: 

                 358     204      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     401     594 
   Size:        401     193
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     243     594 
   Size:        243     351
 
PpuMI rG'GwC_Cy

Cuts at:      0      90     435     594 
   Size:         90     345     159

  Fragments arranged by size: 

                 345     159      90
 
PshAI GACnn'nnGTC

Cuts at:      0      73     594 
   Size:         73     521
 
PspOMI G'GGCC_C

Cuts at:      0     148     594 
   Size:        148     446
 
SapI GCTCTTCn'nnn_

Cuts at:      0      40     594 
   Size:         40     554
 
SmaI CCC'GGG

Cuts at:      0     153     594 
   Size:        153     441
 
SrfI GCCC'GGGC

Cuts at:      0     153     594 
   Size:        153     441
 
StyI C'CwwG_G

Cuts at:      0     242     594 
   Size:        242     352
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     445     594 
   Size:        445     149
 
XbaI T'CTAG_A

Cuts at:      0     252     594 
   Size:        252     342
 
XmaI C'CCGG_G

Cuts at:      0     151     594 
   Size:        151     443
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     AvaI     BanI    BanII     BbsI    BglII
 Bme1580I     BpmI    BsaWI    BseRI     BsgI Bsp1286I    BspEI    BstYI
     EarI     EciI  Eco57MI EcoO109I    Hin4I     MmeI    PflMI    PpuMI
    PshAI   PspOMI     SapI     SmaI     SrfI     StyI    TaqII     XbaI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XcmI
     XhoI     XmnI     ZraI