(Linear) (Six-base) MAPSORT of: KIBB6875.seq  Check: 4605  from: 1  to: 477



KIBB6875.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

Acc65I G'GTAC_C

Cuts at:      0     165     477 
   Size:        165     312
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     477 
   Size:         42     435
 
AfeI AGC'GCT

Cuts at:      0     310     477 
   Size:        310     167
 
AhdI GACnn_n'nnGTC

Cuts at:      0     136     477 
   Size:        136     341
 
AlwNI CAG_nnn'CTG

Cuts at:      0      65     477 
   Size:         65     412
 
ApoI r'AATT_y

Cuts at:      0     454     477 
   Size:        454      23
 
BanI G'GyrC_C

Cuts at:      0     165     477 
   Size:        165     312
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      88     477 
   Size:         88     389
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     112     477 
   Size:        112     365
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     280     477 
   Size:        238      42     197

  Fragments arranged by size: 

                 238     197      42
 
Bpu10I CC'TnA_GC

Cuts at:      0     283     477 
   Size:        283     194
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     238     477 
   Size:        238     239
 
BsaHI Gr'CG_yC

Cuts at:      0     440     477 
   Size:        440      37
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     369     477 
   Size:        369     108
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0       4     477 
   Size:          4     473
 
BsrBI CCG'CTC

Cuts at:      0     326     477 
   Size:        326     151
 
BstEII G'GTnAC_C

Cuts at:      0     447     477 
   Size:        447      30
 
BtgI C'CryG_G

Cuts at:      0     201     477 
   Size:        201     276
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     406     477 
   Size:        406      71
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     333     368     477 
   Size:        333      35     109

  Fragments arranged by size: 

                 333     109      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     409     439     477 
   Size:        409      30      38

  Fragments arranged by size: 

                 409      38      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     238     280     477 
   Size:         42     196      42     197

  Fragments arranged by size: 

                 197     196      42      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     434     477 
   Size:        434      43
 
HaeII r_GCGC'y

Cuts at:      0     312     477 
   Size:        312     165
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      75     107     122     154     477 
   Size:         75      32      15      32     323

  Fragments arranged by size: 

                 323      75      32      32      15
 
HincII GTy'rAC

Cuts at:      0      75     270     477 
   Size:         75     195     207

  Fragments arranged by size: 

                 207     195      75
 
HpaI GTT'AAC

Cuts at:      0      75     270     477 
   Size:         75     195     207

  Fragments arranged by size: 

                 207     195      75
 
KpnI G_GTAC'C

Cuts at:      0     169     477 
   Size:        169     308
 
MslI CAynn'nnrTG

Cuts at:      0     391     424     477 
   Size:        391      33      53

  Fragments arranged by size: 

                 391      53      33
 
MspA1I CmG'CkG

Cuts at:      0     258     477 
   Size:        258     219
 
NcoI C'CATG_G

Cuts at:      0     201     477 
   Size:        201     276
 
PfoI T'CCnGG_A

Cuts at:      0     464     477 
   Size:        464      13
 
PvuII CAG'CTG

Cuts at:      0     258     477 
   Size:        258     219
 
SpeI A'CTAG_T

Cuts at:      0     108     477 
   Size:        108     369
 
StyI C'CwwG_G

Cuts at:      0     201     477 
   Size:        201     276
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     255     477 
   Size:        255     222
 
XmnI GAAnn'nnTTC

Cuts at:      0      85     477 
   Size:         85     392
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI     AhdI    AlwNI     ApoI     BanI     BbsI
    BciVI     BpmI   Bpu10I     BsaI    BsaHI     BsgI    BsmBI    BsrBI
   BstEII     BtgI    BtgZI    CspCI     DrdI  Eco57MI    EcoNI    HaeII
    Hin4I   HincII     HpaI     KpnI     MslI   MspA1I     NcoI     PfoI
    PvuII     SpeI     StyI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII   AflIII     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I     BtsI     ClaI     DraI   DraIII     EaeI
     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     KasI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI