Restriction map for
pF1KB6879
(Linear) (Six-base) MAPSORT of: KIBB6879.seq Check: 8697 from: 1 to: 540
KIBB6879.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 162 540
Size: 162 378
ApaI G_GGCC'C
Cuts at: 0 31 540
Size: 31 509
AvaI C'yCGr_G
Cuts at: 0 101 540
Size: 101 439
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 431 464 540
Size: 431 33 76
Fragments arranged by size:
431 76 33
BamHI G'GATC_C
Cuts at: 0 264 540
Size: 264 276
BanII G_rGCy'C
Cuts at: 0 31 540
Size: 31 509
BbsI GAAGACnn'nnnn_
Cuts at: 0 142 402 540
Size: 142 260 138
Fragments arranged by size:
260 142 138
BglI GCCn_nnn'nGGC
Cuts at: 0 180 296 540
Size: 180 116 244
Fragments arranged by size:
244 180 116
Bme1580I G_kGCm'C
Cuts at: 0 31 540
Size: 31 509
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 326 540
Size: 326 214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 459 540
Size: 184 275 81
Fragments arranged by size:
275 184 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 297 540
Size: 297 243
BsaWI w'CCGG_w
Cuts at: 0 312 540
Size: 312 228
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 457 487 540
Size: 457 30 53
Fragments arranged by size:
457 53 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 31 540
Size: 28 3 509
Fragments arranged by size:
509 28 3
BseYI C'CCAG_C
Cuts at: 0 205 468 540
Size: 205 263 72
Fragments arranged by size:
263 205 72
Bsp1286I G_dGCh'C
Cuts at: 0 31 540
Size: 31 509
BspEI T'CCGG_A
Cuts at: 0 312 540
Size: 312 228
BsrBI CCG'CTC
Cuts at: 0 51 540
Size: 51 489
BstBI TT'CG_AA
Cuts at: 0 74 540
Size: 74 466
BstEII G'GTnAC_C
Cuts at: 0 463 540
Size: 463 77
BstXI CCAn_nnnn'nTGG
Cuts at: 0 340 540
Size: 340 200
BstYI r'GATC_y
Cuts at: 0 264 450 540
Size: 264 186 90
Fragments arranged by size:
264 186 90
EaeI y'GGCC_r
Cuts at: 0 126 142 172 540
Size: 126 16 30 368
Fragments arranged by size:
368 126 30 16
EarI CTCTTCn'nnn_
Cuts at: 0 499 540
Size: 499 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 421 540
Size: 421 119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 459 540
Size: 184 275 81
Fragments arranged by size:
275 184 81
EcoO109I rG'GnC_Cy
Cuts at: 0 27 28 540
Size: 27 1 512
Fragments arranged by size:
512 27 1
MscI TGG'CCA
Cuts at: 0 128 144 540
Size: 128 16 396
Fragments arranged by size:
396 128 16
MspA1I CmG'CkG
Cuts at: 0 10 479 540
Size: 10 469 61
Fragments arranged by size:
469 61 10
NruI TCG'CGA
Cuts at: 0 47 540
Size: 47 493
PasI CC'CwG_GG
Cuts at: 0 294 540
Size: 294 246
PspOMI G'GGCC_C
Cuts at: 0 27 540
Size: 27 513
PvuII CAG'CTG
Cuts at: 0 479 540
Size: 479 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 482 540
Size: 482 58
TatI w'GTAC_w
Cuts at: 0 134 540
Size: 134 406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 457 489 540
Size: 457 32 51
Fragments arranged by size:
457 51 32
Tth111I GACn'n_nGTC
Cuts at: 0 461 540
Size: 461 79
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 367 476 540
Size: 367 109 64
Fragments arranged by size:
367 109 64
Enzymes that do cut:
AlwNI ApaI AvaI BaeI BamHI BanII BbsI BglI
Bme1580I BplI BpmI BsaI BsaWI BsaXI BseRI BseYI
Bsp1286I BspEI BsrBI BstBI BstEII BstXI BstYI EaeI
EarI EciI Eco57MI EcoO109I MscI MspA1I NruI PasI
PspOMI PvuII TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
XbaI XhoI XmaI XmnI ZraI