(Linear) (Six-base) MAPSORT of: KIBB6881.seq  Check: 7457  from: 1  to: 543



KIBB6881.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:      0     275     543 
   Size:        275     268
 
AlwNI CAG_nnn'CTG

Cuts at:      0     387     543 
   Size:        387     156
 
AvaI C'yCGr_G

Cuts at:      0     308     543 
   Size:        308     235
 
BanII G_rGCy'C

Cuts at:      0     347     543 
   Size:        347     196
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     424     458     543 
   Size:        424      34      85

  Fragments arranged by size: 

                 424      85      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     124     543 
   Size:        124     419
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     543 
   Size:        252     291
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     543 
   Size:         10     533
 
BsaBI GATnn'nnATC

Cuts at:      0      55     543 
   Size:         55     488
 
BseYI C'CCAG_C

Cuts at:      0     426     543 
   Size:        426     117
 
BsiHKAI G_wGCw'C

Cuts at:      0     347     543 
   Size:        347     196
 
BsmI GAATG_Cn'

Cuts at:      0     332     435     543 
   Size:        332     103     108

  Fragments arranged by size: 

                 332     108     103
 
Bsp1286I G_dGCh'C

Cuts at:      0     347     543 
   Size:        347     196
 
BsrDI GCAATG_nn'

Cuts at:      0     288     543 
   Size:        288     255
 
BstYI r'GATC_y

Cuts at:      0     384     543 
   Size:        384     159
 
EaeI y'GGCC_r

Cuts at:      0     207     518     543 
   Size:        207     311      25

  Fragments arranged by size: 

                 311     207      25
 
EarI CTCTTCn'nnn_

Cuts at:      0     308     543 
   Size:        308     235
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     349     543 
   Size:        349     194
 
EcoICRI GAG'CTC

Cuts at:      0     345     543 
   Size:        345     198
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     543 
   Size:        252     291
 
FspI TGC'GCA

Cuts at:      0      55     543 
   Size:         55     488
 
FspAI rTGC'GCAy

Cuts at:      0      55     543 
   Size:         55     488
 
HincII GTy'rAC

Cuts at:      0     276     543 
   Size:        276     267
 
MscI TGG'CCA

Cuts at:      0     209     520     543 
   Size:        209     311      23

  Fragments arranged by size: 

                 311     209      23
 
MslI CAynn'nnrTG

Cuts at:      0      61     543 
   Size:         61     482
 
MspA1I CmG'CkG

Cuts at:      0     485     543 
   Size:        485      58
 
PfoI T'CCnGG_A

Cuts at:      0     347     543 
   Size:        347     196
 
SacI G_AGCT'C

Cuts at:      0     347     543 
   Size:        347     196
 
SalI G'TCGA_C

Cuts at:      0     274     543 
   Size:        274     269
 
SapI GCTCTTCn'nnn_

Cuts at:      0     308     543 
   Size:        308     235
 
SmaI CCC'GGG

Cuts at:      0     310     543 
   Size:        310     233
 
SmlI C'TyrA_G

Cuts at:      0      25     543 
   Size:         25     518
 
StuI AGG'CCT

Cuts at:      0     506     543 
   Size:        506      37
 
StyI C'CwwG_G

Cuts at:      0     255     543 
   Size:        255     288
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     193     268     543 
   Size:        193      75     275

  Fragments arranged by size: 

                 275     193      75
 
TatI w'GTAC_w

Cuts at:      0     357     543 
   Size:        357     186
 
XmaI C'CCGG_G

Cuts at:      0     308     543 
   Size:        308     235
 

 Enzymes that do cut:

     AccI    AlwNI     AvaI    BanII     BcgI     BmrI     BpmI    BpuEI
    BsaBI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI    BstYI     EaeI
     EarI     EciI  EcoICRI  Eco57MI     FspI    FspAI   HincII     MscI
     MslI   MspA1I     PfoI     SacI     SalI     SapI     SmaI     SmlI
     StuI     StyI    TaqII     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI    HaeII    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI