Restriction map for
pF1KB6882
(Linear) (Six-base) MAPSORT of: KIBB6882.seq Check: 1324 from: 1 to: 603
KIBB6882.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 0 374 603
Size: 374 229
AflIII A'CryG_T
Cuts at: 0 257 389 603
Size: 257 132 214
Fragments arranged by size:
257 214 132
AvaI C'yCGr_G
Cuts at: 0 148 467 603
Size: 148 319 136
Fragments arranged by size:
319 148 136
BanI G'GyrC_C
Cuts at: 0 277 433 603
Size: 277 156 170
Fragments arranged by size:
277 170 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 317 376 410 603
Size: 283 34 59 34 193
Fragments arranged by size:
283 193 59 34 34
Bme1580I G_kGCm'C
Cuts at: 0 280 603
Size: 280 323
BmgBI CAC'GTC
Cuts at: 0 258 603
Size: 258 345
BmrI ACTGGGnnnn_n'
Cuts at: 0 261 333 603
Size: 261 72 270
Fragments arranged by size:
270 261 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 603
Size: 328 275
BsaI GGTCTCn'nnnn_
Cuts at: 0 193 603
Size: 193 410
BsaAI yAC'GTr
Cuts at: 0 392 603
Size: 392 211
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 514 544 603
Size: 436 30 48 30 59
Fragments arranged by size:
436 59 48 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 603
Size: 538 65
BseYI C'CCAG_C
Cuts at: 0 36 603
Size: 36 567
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 603
Size: 421 182
BsiEI CG_ry'CG
Cuts at: 0 48 603
Size: 48 555
Bsp1286I G_dGCh'C
Cuts at: 0 280 603
Size: 280 323
BsrFI r'CCGG_y
Cuts at: 0 202 603
Size: 202 401
BtgI C'CryG_G
Cuts at: 0 131 191 603
Size: 131 60 412
Fragments arranged by size:
412 131 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 590 603
Size: 590 13
DrdI GACnn_nn'nnGTC
Cuts at: 0 175 603
Size: 175 428
EaeI y'GGCC_r
Cuts at: 0 45 134 204 536 603
Size: 45 89 70 332 67
Fragments arranged by size:
332 89 70 67 45
EagI C'GGCC_G
Cuts at: 0 45 603
Size: 45 558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 603
Size: 328 275
EcoO109I rG'GnC_Cy
Cuts at: 0 119 144 153 297 471 603
Size: 119 25 9 144 174 132
Fragments arranged by size:
174 144 132 119 25 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 111 126 603
Size: 111 15 477
Fragments arranged by size:
477 111 15
FspI TGC'GCA
Cuts at: 0 264 603
Size: 264 339
HaeII r_GCGC'y
Cuts at: 0 19 37 218 603
Size: 19 18 181 385
Fragments arranged by size:
385 181 19 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 568 600 603
Size: 436 32 100 32 3
Fragments arranged by size:
436 100 32 32 3
HincII GTy'rAC
Cuts at: 0 375 603
Size: 375 228
MscI TGG'CCA
Cuts at: 0 136 538 603
Size: 136 402 65
Fragments arranged by size:
402 136 65
MspA1I CmG'CkG
Cuts at: 0 44 516 603
Size: 44 472 87
Fragments arranged by size:
472 87 44
NcoI C'CATG_G
Cuts at: 0 131 603
Size: 131 472
NotI GC'GGCC_GC
Cuts at: 0 45 603
Size: 45 558
PpuMI rG'GwC_Cy
Cuts at: 0 119 144 297 471 603
Size: 119 25 153 174 132
Fragments arranged by size:
174 153 132 119 25
PstI C_TGCA'G
Cuts at: 0 167 603
Size: 167 436
SalI G'TCGA_C
Cuts at: 0 373 603
Size: 373 230
SanDI GG'GwC_CC
Cuts at: 0 471 603
Size: 471 132
ScaI AGT'ACT
Cuts at: 0 322 406 603
Size: 322 84 197
Fragments arranged by size:
322 197 84
SexAI A'CCwGG_T
Cuts at: 0 121 603
Size: 121 482
SfcI C'TryA_G
Cuts at: 0 163 603
Size: 163 440
SmaI CCC'GGG
Cuts at: 0 469 603
Size: 469 134
StyI C'CwwG_G
Cuts at: 0 131 224 603
Size: 131 93 379
Fragments arranged by size:
379 131 93
TatI w'GTAC_w
Cuts at: 0 320 404 545 603
Size: 320 84 141 58
Fragments arranged by size:
320 141 84 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 514 546 603
Size: 514 32 57
Fragments arranged by size:
514 57 32
XmaI C'CCGG_G
Cuts at: 0 467 603
Size: 467 136
Enzymes that do cut:
AccI AflIII AvaI BanI BcgI Bme1580I BmgBI BmrI
BpmI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiEI
Bsp1286I BsrFI BtgI BtgZI DrdI EaeI EagI Eco57MI
EcoO109I FalI FspI HaeII Hin4I HincII MscI MspA1I
NcoI NotI PpuMI PstI SalI SanDI ScaI SexAI
SfcI SmaI StyI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI
BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsaWI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EarI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NaeI
NarI NdeI NgoMIV NheI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SapI SbfI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmnI ZraI