(Linear) (Six-base) MAPSORT of: KIBB6883.seq Check: 4971 from: 1 to: 543 KIBB6883.orf With 182 enzymes: SgfI * March 2, 2010 14:27 .. ApoI r'AATT_y Cuts at: 0 169 184 530 543 Size: 169 15 346 13 Fragments arranged by size: 346 169 15 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 146 543 Size: 146 397 BglI GCCn_nnn'nGGC Cuts at: 0 112 543 Size: 112 431 BglII A'GATC_T Cuts at: 0 275 398 543 Size: 275 123 145 Fragments arranged by size: 275 145 123 BlpI GC'TnA_GC Cuts at: 0 25 543 Size: 25 518 BmtI G_CTAG'C Cuts at: 0 217 543 Size: 217 326 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 373 543 Size: 145 228 170 Fragments arranged by size: 228 170 145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 543 Size: 79 464 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 316 346 543 Size: 316 30 197 Fragments arranged by size: 316 197 30 BsiEI CG_ry'CG Cuts at: 0 40 543 Size: 40 503 BsiHKAI G_wGCw'C Cuts at: 0 103 543 Size: 103 440 Bsp1286I G_dGCh'C Cuts at: 0 103 543 Size: 103 440 BspHI T'CATG_A Cuts at: 0 410 543 Size: 410 133 BsrFI r'CCGG_y Cuts at: 0 20 543 Size: 20 523 BstYI r'GATC_y Cuts at: 0 275 350 398 543 Size: 275 75 48 145 Fragments arranged by size: 275 145 75 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 77 543 Size: 77 466 DraI TTT'AAA Cuts at: 0 176 458 543 Size: 176 282 85 Fragments arranged by size: 282 176 85 DrdI GACnn_nn'nnGTC Cuts at: 0 291 543 Size: 291 252 EaeI y'GGCC_r Cuts at: 0 37 543 Size: 37 506 EagI C'GGCC_G Cuts at: 0 37 543 Size: 37 506 EarI CTCTTCn'nnn_ Cuts at: 0 473 543 Size: 473 70 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 145 373 543 Size: 145 228 170 Fragments arranged by size: 228 170 145 EcoRI G'AATT_C Cuts at: 0 184 543 Size: 184 359 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 465 543 Size: 465 78 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 316 348 390 422 543 Size: 316 32 42 32 121 Fragments arranged by size: 316 121 42 32 32 HincII GTy'rAC Cuts at: 0 318 543 Size: 318 225 NaeI GCC'GGC Cuts at: 0 22 543 Size: 22 521 NgoMIV G'CCGG_C Cuts at: 0 20 543 Size: 20 523 NheI G'CTAG_C Cuts at: 0 213 543 Size: 213 330 NotI GC'GGCC_GC Cuts at: 0 37 543 Size: 37 506 NspI r_CATG'y Cuts at: 0 221 543 Size: 221 322 PfoI T'CCnGG_A Cuts at: 0 353 389 543 Size: 353 36 154 Fragments arranged by size: 353 154 36 PsiI TTA'TAA Cuts at: 0 536 543 Size: 536 7 PstI C_TGCA'G Cuts at: 0 513 543 Size: 513 30 SfcI C'TryA_G Cuts at: 0 426 509 543 Size: 426 83 34 Fragments arranged by size: 426 83 34 SmlI C'TyrA_G Cuts at: 0 94 543 Size: 94 449 StuI AGG'CCT Cuts at: 0 304 543 Size: 304 239 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 61 543 Size: 61 482 Enzymes that do cut: ApoI BbsI BglI BglII BlpI BmtI BpmI BpuEI BsaXI BsiEI BsiHKAI Bsp1286I BspHI BsrFI BstYI BtgZI DraI DrdI EaeI EagI EarI Eco57MI EcoRI FalI Hin4I HincII NaeI NgoMIV NheI NotI NspI PfoI PsiI PstI SfcI SmlI StuI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI Bme1580I BmgBI BmrI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraIII EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI