(Linear) (Six-base) MAPSORT of: KIBB6883.seq  Check: 4971  from: 1  to: 543



KIBB6883.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

ApoI r'AATT_y

Cuts at:      0     169     184     530     543 
   Size:        169      15     346      13

  Fragments arranged by size: 

                 346     169      15      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     146     543 
   Size:        146     397
 
BglI GCCn_nnn'nGGC

Cuts at:      0     112     543 
   Size:        112     431
 
BglII A'GATC_T

Cuts at:      0     275     398     543 
   Size:        275     123     145

  Fragments arranged by size: 

                 275     145     123
 
BlpI GC'TnA_GC

Cuts at:      0      25     543 
   Size:         25     518
 
BmtI G_CTAG'C

Cuts at:      0     217     543 
   Size:        217     326
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     373     543 
   Size:        145     228     170

  Fragments arranged by size: 

                 228     170     145
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     543 
   Size:         79     464
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     316     346     543 
   Size:        316      30     197

  Fragments arranged by size: 

                 316     197      30
 
BsiEI CG_ry'CG

Cuts at:      0      40     543 
   Size:         40     503
 
BsiHKAI G_wGCw'C

Cuts at:      0     103     543 
   Size:        103     440
 
Bsp1286I G_dGCh'C

Cuts at:      0     103     543 
   Size:        103     440
 
BspHI T'CATG_A

Cuts at:      0     410     543 
   Size:        410     133
 
BsrFI r'CCGG_y

Cuts at:      0      20     543 
   Size:         20     523
 
BstYI r'GATC_y

Cuts at:      0     275     350     398     543 
   Size:        275      75      48     145

  Fragments arranged by size: 

                 275     145      75      48
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      77     543 
   Size:         77     466
 
DraI TTT'AAA

Cuts at:      0     176     458     543 
   Size:        176     282      85

  Fragments arranged by size: 

                 282     176      85
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     291     543 
   Size:        291     252
 
EaeI y'GGCC_r

Cuts at:      0      37     543 
   Size:         37     506
 
EagI C'GGCC_G

Cuts at:      0      37     543 
   Size:         37     506
 
EarI CTCTTCn'nnn_

Cuts at:      0     473     543 
   Size:        473      70
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     373     543 
   Size:        145     228     170

  Fragments arranged by size: 

                 228     170     145
 
EcoRI G'AATT_C

Cuts at:      0     184     543 
   Size:        184     359
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     465     543 
   Size:        465      78
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     316     348     390     422     543 
   Size:        316      32      42      32     121

  Fragments arranged by size: 

                 316     121      42      32      32
 
HincII GTy'rAC

Cuts at:      0     318     543 
   Size:        318     225
 
NaeI GCC'GGC

Cuts at:      0      22     543 
   Size:         22     521
 
NgoMIV G'CCGG_C

Cuts at:      0      20     543 
   Size:         20     523
 
NheI G'CTAG_C

Cuts at:      0     213     543 
   Size:        213     330
 
NotI GC'GGCC_GC

Cuts at:      0      37     543 
   Size:         37     506
 
NspI r_CATG'y

Cuts at:      0     221     543 
   Size:        221     322
 
PfoI T'CCnGG_A

Cuts at:      0     353     389     543 
   Size:        353      36     154

  Fragments arranged by size: 

                 353     154      36
 
PsiI TTA'TAA

Cuts at:      0     536     543 
   Size:        536       7
 
PstI C_TGCA'G

Cuts at:      0     513     543 
   Size:        513      30
 
SfcI C'TryA_G

Cuts at:      0     426     509     543 
   Size:        426      83      34

  Fragments arranged by size: 

                 426      83      34
 
SmlI C'TyrA_G

Cuts at:      0      94     543 
   Size:         94     449
 
StuI AGG'CCT

Cuts at:      0     304     543 
   Size:        304     239
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      61     543 
   Size:         61     482
 

 Enzymes that do cut:

     ApoI     BbsI     BglI    BglII     BlpI     BmtI     BpmI    BpuEI
    BsaXI    BsiEI  BsiHKAI Bsp1286I    BspHI    BsrFI    BstYI    BtgZI
     DraI     DrdI     EaeI     EagI     EarI  Eco57MI    EcoRI     FalI
    Hin4I   HincII     NaeI   NgoMIV     NheI     NotI     NspI     PfoI
     PsiI     PstI     SfcI     SmlI     StuI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
 Bme1580I    BmgBI     BmrI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI
    CspCI   DraIII     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI