Restriction map for
pF1KB6884
(Linear) (Six-base) MAPSORT of: KIBB6884.seq Check: 5598 from: 1 to: 603
KIBB6884.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 267 603
Size: 267 336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 313 514 603
Size: 187 126 201 89
Fragments arranged by size:
201 187 126 89
AflIII A'CryG_T
Cuts at: 0 329 603
Size: 329 274
AleI CACnn'nnGTG
Cuts at: 0 434 603
Size: 434 169
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 235 267 603
Size: 235 32 336
Fragments arranged by size:
336 235 32
AlwNI CAG_nnn'CTG
Cuts at: 0 291 603
Size: 291 312
ApoI r'AATT_y
Cuts at: 0 484 603
Size: 484 119
AvaI C'yCGr_G
Cuts at: 0 238 603
Size: 238 365
BanI G'GyrC_C
Cuts at: 0 234 603
Size: 234 369
BbsI GAAGACnn'nnnn_
Cuts at: 0 252 603
Size: 252 351
BbvCI CC'TCA_GC
Cuts at: 0 302 603
Size: 302 301
BglI GCCn_nnn'nGGC
Cuts at: 0 559 603
Size: 559 44
Bme1580I G_kGCm'C
Cuts at: 0 239 431 603
Size: 239 192 172
Fragments arranged by size:
239 192 172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 603
Size: 97 506
Bpu10I CC'TnA_GC
Cuts at: 0 302 603
Size: 302 301
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 235 265 603
Size: 235 30 338
Fragments arranged by size:
338 235 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 94 603
Size: 85 9 509
Fragments arranged by size:
509 85 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 127 397 603
Size: 61 66 270 206
Fragments arranged by size:
270 206 66 61
Bsp1286I G_dGCh'C
Cuts at: 0 239 431 603
Size: 239 192 172
Fragments arranged by size:
239 192 172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 267 603
Size: 267 336
BsrBI CCG'CTC
Cuts at: 0 84 603
Size: 84 519
BsrFI r'CCGG_y
Cuts at: 0 225 562 603
Size: 225 337 41
Fragments arranged by size:
337 225 41
BstYI r'GATC_y
Cuts at: 0 441 603
Size: 441 162
BtgI C'CryG_G
Cuts at: 0 138 254 435 603
Size: 138 116 181 168
Fragments arranged by size:
181 168 138 116
BtsI GCAGTG_nn'
Cuts at: 0 115 380 416 603
Size: 115 265 36 187
Fragments arranged by size:
265 187 115 36
DrdI GACnn_nn'nnGTC
Cuts at: 0 313 603
Size: 313 290
EaeI y'GGCC_r
Cuts at: 0 2 603
Size: 2 601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 187 313 514 603
Size: 97 90 126 201 89
Fragments arranged by size:
201 126 97 90 89
EcoO109I rG'GnC_Cy
Cuts at: 0 13 383 551 603
Size: 13 370 168 52
Fragments arranged by size:
370 168 52 13
EcoRI G'AATT_C
Cuts at: 0 484 603
Size: 484 119
HaeII r_GCGC'y
Cuts at: 0 147 603
Size: 147 456
HincII GTy'rAC
Cuts at: 0 345 603
Size: 345 258
MscI TGG'CCA
Cuts at: 0 4 603
Size: 4 599
MslI CAynn'nnrTG
Cuts at: 0 434 603
Size: 434 169
MspA1I CmG'CkG
Cuts at: 0 501 603
Size: 501 102
NaeI GCC'GGC
Cuts at: 0 564 603
Size: 564 39
NcoI C'CATG_G
Cuts at: 0 254 603
Size: 254 349
NgoMIV G'CCGG_C
Cuts at: 0 562 603
Size: 562 41
NspI r_CATG'y
Cuts at: 0 333 520 603
Size: 333 187 83
Fragments arranged by size:
333 187 83
PciI A'CATG_T
Cuts at: 0 329 603
Size: 329 274
PflMI CCAn_nnn'nTGG
Cuts at: 0 139 603
Size: 139 464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 603
Size: 235 32 336
Fragments arranged by size:
336 235 32
PpuMI rG'GwC_Cy
Cuts at: 0 13 383 603
Size: 13 370 220
Fragments arranged by size:
370 220 13
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 190 222 603
Size: 190 32 381
Fragments arranged by size:
381 190 32
PstI C_TGCA'G
Cuts at: 0 506 603
Size: 506 97
PvuII CAG'CTG
Cuts at: 0 501 603
Size: 501 102
SfcI C'TryA_G
Cuts at: 0 502 603
Size: 502 101
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 559 603
Size: 559 44
StyI C'CwwG_G
Cuts at: 0 254 603
Size: 254 349
Enzymes that do cut:
AarI AcuI AflIII AleI AloI AlwNI ApoI AvaI
BanI BbsI BbvCI BglI Bme1580I BpmI Bpu10I BsaXI
BseRI BsgI Bsp1286I BspMI BsrBI BsrFI BstYI BtgI
BtsI DrdI EaeI Eco57MI EcoO109I EcoRI HaeII HincII
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PciI
PflMI PpiI PpuMI PsrI PstI PvuII SfcI SfiI
StyI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EagI EarI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI Hin4I HindIII HpaI KasI
KpnI MfeI MluI MmeI NarI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI