Restriction map for
pF1KB6885
(Linear) (Six-base) MAPSORT of: KIBB6885.seq Check: 5009 from: 1 to: 540
KIBB6885.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 0 283 540
Size: 283 257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 540
Size: 138 402
AflIII A'CryG_T
Cuts at: 0 278 540
Size: 278 262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 403 540
Size: 403 137
AlwNI CAG_nnn'CTG
Cuts at: 0 375 540
Size: 375 165
AvaI C'yCGr_G
Cuts at: 0 436 540
Size: 436 104
BanI G'GyrC_C
Cuts at: 0 508 540
Size: 508 32
BanII G_rGCy'C
Cuts at: 0 437 540
Size: 437 103
BbeI G_GCGC'C
Cuts at: 0 512 540
Size: 512 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 12 46 540
Size: 12 34 494
Fragments arranged by size:
494 34 12
Bme1580I G_kGCm'C
Cuts at: 0 408 540
Size: 408 132
BmgBI CAC'GTC
Cuts at: 0 279 540
Size: 279 261
BmrI ACTGGGnnnn_n'
Cuts at: 0 77 540
Size: 77 463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 259 540
Size: 106 153 281
Fragments arranged by size:
281 153 106
BsaI GGTCTCn'nnnn_
Cuts at: 0 82 540
Size: 82 458
BsaAI yAC'GTr
Cuts at: 0 420 540
Size: 420 120
BsaHI Gr'CG_yC
Cuts at: 0 509 540
Size: 509 31
BsaWI w'CCGG_w
Cuts at: 0 303 540
Size: 303 237
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 540
Size: 217 323
BseYI C'CCAG_C
Cuts at: 0 368 540
Size: 368 172
BsiEI CG_ry'CG
Cuts at: 0 296 540
Size: 296 244
BsiHKAI G_wGCw'C
Cuts at: 0 425 437 540
Size: 425 12 103
Fragments arranged by size:
425 103 12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 235 540
Size: 235 305
Bsp1286I G_dGCh'C
Cuts at: 0 408 425 437 540
Size: 408 17 12 103
Fragments arranged by size:
408 103 17 12
BspEI T'CCGG_A
Cuts at: 0 303 540
Size: 303 237
BsrDI GCAATG_nn'
Cuts at: 0 65 540
Size: 65 475
BssSI C'ACGA_G
Cuts at: 0 57 540
Size: 57 483
BtgI C'CryG_G
Cuts at: 0 141 189 326 501 540
Size: 141 48 137 175 39
Fragments arranged by size:
175 141 137 48 39
EaeI y'GGCC_r
Cuts at: 0 524 540
Size: 524 16
EcoICRI GAG'CTC
Cuts at: 0 435 540
Size: 435 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 138 259 540
Size: 106 32 121 281
Fragments arranged by size:
281 121 106 32
EcoO109I rG'GnC_Cy
Cuts at: 0 384 540
Size: 384 156
FspI TGC'GCA
Cuts at: 0 379 540
Size: 379 161
HaeII r_GCGC'y
Cuts at: 0 375 512 540
Size: 375 137 28
Fragments arranged by size:
375 137 28
HincII GTy'rAC
Cuts at: 0 284 540
Size: 284 256
KasI G'GCGC_C
Cuts at: 0 508 540
Size: 508 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 540
Size: 316 224
MscI TGG'CCA
Cuts at: 0 526 540
Size: 526 14
MspA1I CmG'CkG
Cuts at: 0 143 221 540
Size: 143 78 319
Fragments arranged by size:
319 143 78
NarI GG'CG_CC
Cuts at: 0 509 540
Size: 509 31
PfoI T'CCnGG_A
Cuts at: 0 236 540
Size: 236 304
PmlI CAC'GTG
Cuts at: 0 420 540
Size: 420 120
PspXI vC'TCGA_Gb
Cuts at: 0 436 540
Size: 436 104
PvuII CAG'CTG
Cuts at: 0 221 540
Size: 221 319
SacI G_AGCT'C
Cuts at: 0 437 540
Size: 437 103
SacII CC_GC'GG
Cuts at: 0 144 540
Size: 144 396
SalI G'TCGA_C
Cuts at: 0 282 540
Size: 282 258
SfoI GGC'GCC
Cuts at: 0 510 540
Size: 510 30
SmlI C'TyrA_G
Cuts at: 0 436 540
Size: 436 104
StyI C'CwwG_G
Cuts at: 0 428 540
Size: 428 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 382 502 540
Size: 382 120 38
Fragments arranged by size:
382 120 38
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 130 324 540
Size: 130 194 216
Fragments arranged by size:
216 194 130
XhoI C'TCGA_G
Cuts at: 0 436 540
Size: 436 104
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI AvaI BanI BanII
BbeI BcgI Bme1580I BmgBI BmrI BpmI BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BsrDI BssSI BtgI EaeI EcoICRI Eco57MI EcoO109I
FspI HaeII HincII KasI MmeI MscI MspA1I NarI
PfoI PmlI PspXI PvuII SacI SacII SalI SfoI
SmlI StyI TaqII XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaBI
BsaXI BsgI BsiWI BsmI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspAI Hin4I HindIII HpaI KpnI MfeI MluI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI RsrII SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XmaI XmnI ZraI