Restriction map for
pF1KB6886
(Linear) (Six-base) MAPSORT of: KIBB6886.seq Check: 5104 from: 1 to: 573
KIBB6886.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 562 573
Size: 249 313 11
Fragments arranged by size:
313 249 11
AlwNI CAG_nnn'CTG
Cuts at: 0 84 292 573
Size: 84 208 281
Fragments arranged by size:
281 208 84
ApoI r'AATT_y
Cuts at: 0 102 573
Size: 102 471
BanI G'GyrC_C
Cuts at: 0 21 573
Size: 21 552
BanII G_rGCy'C
Cuts at: 0 65 404 573
Size: 65 339 169
Fragments arranged by size:
339 169 65
BbeI G_GCGC'C
Cuts at: 0 25 573
Size: 25 548
BbsI GAAGACnn'nnnn_
Cuts at: 0 152 573
Size: 152 421
BbvCI CC'TCA_GC
Cuts at: 0 285 573
Size: 285 288
BglII A'GATC_T
Cuts at: 0 116 573
Size: 116 457
BlpI GC'TnA_GC
Cuts at: 0 254 573
Size: 254 319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 573
Size: 359 214
Bpu10I CC'TnA_GC
Cuts at: 0 285 573
Size: 285 288
BsaBI GATnn'nnATC
Cuts at: 0 188 573
Size: 188 385
BsaHI Gr'CG_yC
Cuts at: 0 22 573
Size: 22 551
BsaWI w'CCGG_w
Cuts at: 0 236 573
Size: 236 337
BseYI C'CCAG_C
Cuts at: 0 179 403 573
Size: 179 224 170
Fragments arranged by size:
224 179 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 573
Size: 460 113
BsiHKAI G_wGCw'C
Cuts at: 0 65 573
Size: 65 508
Bsp1286I G_dGCh'C
Cuts at: 0 65 404 573
Size: 65 339 169
Fragments arranged by size:
339 169 65
BsrBI CCG'CTC
Cuts at: 0 258 573
Size: 258 315
BsrFI r'CCGG_y
Cuts at: 0 524 573
Size: 524 49
BssSI C'ACGA_G
Cuts at: 0 64 560 573
Size: 64 496 13
Fragments arranged by size:
496 64 13
BstAPI GCAn_nnn'nTGC
Cuts at: 0 301 573
Size: 301 272
BstEII G'GTnAC_C
Cuts at: 0 279 573
Size: 279 294
BstYI r'GATC_y
Cuts at: 0 32 116 423 433 573
Size: 32 84 307 10 140
Fragments arranged by size:
307 140 84 32 10
Bsu36I CC'TnA_GG
Cuts at: 0 419 480 573
Size: 419 61 93
Fragments arranged by size:
419 93 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 19 573
Size: 19 554
EarI CTCTTCn'nnn_
Cuts at: 0 268 573
Size: 268 305
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 73 103 573
Size: 73 30 470
Fragments arranged by size:
470 73 30
EcoICRI GAG'CTC
Cuts at: 0 63 573
Size: 63 510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 359 562 573
Size: 249 110 203 11
Fragments arranged by size:
249 203 110 11
EcoO109I rG'GnC_Cy
Cuts at: 0 414 573
Size: 414 159
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 386 573
Size: 386 187
HaeII r_GCGC'y
Cuts at: 0 25 573
Size: 25 548
KasI G'GCGC_C
Cuts at: 0 21 573
Size: 21 552
MslI CAynn'nnrTG
Cuts at: 0 154 573
Size: 154 419
MspA1I CmG'CkG
Cuts at: 0 292 573
Size: 292 281
NarI GG'CG_CC
Cuts at: 0 22 573
Size: 22 551
PflMI CCAn_nnn'nTGG
Cuts at: 0 352 573
Size: 352 221
PfoI T'CCnGG_A
Cuts at: 0 35 573
Size: 35 538
PpuMI rG'GwC_Cy
Cuts at: 0 414 573
Size: 414 159
PstI C_TGCA'G
Cuts at: 0 297 573
Size: 297 276
PvuII CAG'CTG
Cuts at: 0 292 573
Size: 292 281
SacI G_AGCT'C
Cuts at: 0 65 573
Size: 65 508
SapI GCTCTTCn'nnn_
Cuts at: 0 268 573
Size: 268 305
SfcI C'TryA_G
Cuts at: 0 293 573
Size: 293 280
SfoI GGC'GCC
Cuts at: 0 23 573
Size: 23 550
SspI AAT'ATT
Cuts at: 0 168 573
Size: 168 405
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 348 380 573
Size: 348 32 193
Fragments arranged by size:
348 193 32
Enzymes that do cut:
AcuI AlwNI ApoI BanI BanII BbeI BbsI BbvCI
BglII BlpI BpmI Bpu10I BsaBI BsaHI BsaWI BseYI
BsgI BsiHKAI Bsp1286I BsrBI BsrFI BssSI BstAPI BstEII
BstYI Bsu36I BtgZI EarI EciI EcoICRI Eco57MI EcoO109I
FalI HaeII KasI MslI MspA1I NarI PflMI PfoI
PpuMI PstI PvuII SacI SapI SfcI SfoI SspI
TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BcgI BciVI
BclI BfrBI BglI Bme1580I BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaXI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BstBI
BstXI BstZ17I BtgI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EcoNI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI