Restriction map for
pF1KB6890
(Linear) (Six-base) MAPSORT of: KIBB6890.seq Check: 6589 from: 1 to: 606
KIBB6890.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 301 606
Size: 301 305
AflIII A'CryG_T
Cuts at: 0 209 606
Size: 209 397
AlwNI CAG_nnn'CTG
Cuts at: 0 259 606
Size: 259 347
ApaI G_GGCC'C
Cuts at: 0 190 606
Size: 190 416
ApoI r'AATT_y
Cuts at: 0 154 606
Size: 154 452
AvaI C'yCGr_G
Cuts at: 0 42 431 606
Size: 42 389 175
Fragments arranged by size:
389 175 42
BanII G_rGCy'C
Cuts at: 0 49 190 557 606
Size: 49 141 367 49
Fragments arranged by size:
367 141 49 49
BclI T'GATC_A
Cuts at: 0 159 606
Size: 159 447
Bme1580I G_kGCm'C
Cuts at: 0 190 606
Size: 190 416
BmrI ACTGGGnnnn_n'
Cuts at: 0 465 606
Size: 465 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 133 388 499 606
Size: 55 78 255 111 107
Fragments arranged by size:
255 111 107 78 55
Bpu10I CC'TnA_GC
Cuts at: 0 359 606
Size: 359 247
BsaI GGTCTCn'nnnn_
Cuts at: 0 484 606
Size: 484 122
BsaHI Gr'CG_yC
Cuts at: 0 230 606
Size: 230 376
BsaWI w'CCGG_w
Cuts at: 0 65 606
Size: 65 541
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 568 606
Size: 568 38
BseYI C'CCAG_C
Cuts at: 0 398 606
Size: 398 208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 279 516 606
Size: 108 171 237 90
Fragments arranged by size:
237 171 108 90
Bsp1286I G_dGCh'C
Cuts at: 0 49 190 557 606
Size: 49 141 367 49
Fragments arranged by size:
367 141 49 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 301 606
Size: 301 305
BstEII G'GTnAC_C
Cuts at: 0 435 606
Size: 435 171
BstYI r'GATC_y
Cuts at: 0 164 606
Size: 164 442
BtgI C'CryG_G
Cuts at: 0 266 303 593 606
Size: 266 37 290 13
Fragments arranged by size:
290 266 37 13
BtsI GCAGTG_nn'
Cuts at: 0 96 606
Size: 96 510
EaeI y'GGCC_r
Cuts at: 0 2 606
Size: 2 604
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 118 606
Size: 118 488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 133 388 499 606
Size: 55 78 255 111 107
Fragments arranged by size:
255 111 107 78 55
EcoO109I rG'GnC_Cy
Cuts at: 0 187 498 606
Size: 187 311 108
Fragments arranged by size:
311 187 108
HincII GTy'rAC
Cuts at: 0 339 606
Size: 339 267
MscI TGG'CCA
Cuts at: 0 4 606
Size: 4 602
MslI CAynn'nnrTG
Cuts at: 0 322 606
Size: 322 284
MspA1I CmG'CkG
Cuts at: 0 408 606
Size: 408 198
NcoI C'CATG_G
Cuts at: 0 303 593 606
Size: 303 290 13
Fragments arranged by size:
303 290 13
NspI r_CATG'y
Cuts at: 0 213 606
Size: 213 393
PasI CC'CwG_GG
Cuts at: 0 507 606
Size: 507 99
PciI A'CATG_T
Cuts at: 0 209 606
Size: 209 397
PfoI T'CCnGG_A
Cuts at: 0 32 606
Size: 32 574
PpuMI rG'GwC_Cy
Cuts at: 0 498 606
Size: 498 108
PshAI GACnn'nnGTC
Cuts at: 0 280 606
Size: 280 326
PspOMI G'GGCC_C
Cuts at: 0 186 606
Size: 186 420
SmaI CCC'GGG
Cuts at: 0 433 606
Size: 433 173
StyI C'CwwG_G
Cuts at: 0 5 303 593 606
Size: 5 298 290 13
Fragments arranged by size:
298 290 13 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 259 606
Size: 259 347
XmaI C'CCGG_G
Cuts at: 0 431 606
Size: 431 175
Enzymes that do cut:
AarI AflIII AlwNI ApaI ApoI AvaI BanII BclI
Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BsaWI BseRI
BseYI BsgI Bsp1286I BspMI BstEII BstYI BtgI BtsI
EaeI EciI Eco57MI EcoO109I HincII MscI MslI MspA1I
NcoI NspI PasI PciI PfoI PpuMI PshAI PspOMI
SmaI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaXI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PflMI PmeI PmlI PpiI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI