(Linear) (Six-base) MAPSORT of: KIBB6891.seq  Check: 8220  from: 1  to: 549



KIBB6891.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      12     414     549 
   Size:         12     402     135

  Fragments arranged by size: 

                 402     135      12
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     549 
   Size:        232     317
 
BanII G_rGCy'C

Cuts at:      0      86     549 
   Size:         86     463
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     288     549 
   Size:        288     261
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     549 
   Size:        100     449
 
BsaAI yAC'GTr

Cuts at:      0     456     549 
   Size:        456      93
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     199     549 
   Size:        199     350
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     549 
   Size:        337     212
 
BsiEI CG_ry'CG

Cuts at:      0       8     549 
   Size:          8     541
 
BsiHKAI G_wGCw'C

Cuts at:      0      86     549 
   Size:         86     463
 
BsmI GAATG_Cn'

Cuts at:      0     300     549 
   Size:        300     249
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     549 
   Size:         86     463
 
BspHI T'CATG_A

Cuts at:      0     180     549 
   Size:        180     369
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      12     414     549 
   Size:         12     402     135

  Fragments arranged by size: 

                 402     135      12
 
BsrBI CCG'CTC

Cuts at:      0     287     549 
   Size:        287     262
 
BsrDI GCAATG_nn'

Cuts at:      0     305     549 
   Size:        305     244
 
BstEII G'GTnAC_C

Cuts at:      0     448     549 
   Size:        448     101
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     232     549 
   Size:        232     317
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     146     549 
   Size:        146     403
 
DrdI GACnn_nn'nnGTC

Cuts at:      0       9     549 
   Size:          9     540
 
EaeI y'GGCC_r

Cuts at:      0     234     362     549 
   Size:        234     128     187

  Fragments arranged by size: 

                 234     187     128
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     514     549 
   Size:        514      35
 
EcoICRI GAG'CTC

Cuts at:      0      84     549 
   Size:         84     465
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     232     549 
   Size:        100     132     317

  Fragments arranged by size: 

                 317     132     100
 
EcoRV GAT'ATC

Cuts at:      0     255     549 
   Size:        255     294
 
FspI TGC'GCA

Cuts at:      0     469     549 
   Size:        469      80
 
HaeII r_GCGC'y

Cuts at:      0     484     549 
   Size:        484      65
 
MscI TGG'CCA

Cuts at:      0     364     549 
   Size:        364     185
 
MslI CAynn'nnrTG

Cuts at:      0     389     549 
   Size:        389     160
 
NspI r_CATG'y

Cuts at:      0     199     549 
   Size:        199     350
 
PasI CC'CwG_GG

Cuts at:      0     525     549 
   Size:        525      24
 
PmlI CAC'GTG

Cuts at:      0     456     549 
   Size:        456      93
 
PstI C_TGCA'G

Cuts at:      0     221     549 
   Size:        221     328
 
SacI G_AGCT'C

Cuts at:      0      86     549 
   Size:         86     463
 
SfcI C'TryA_G

Cuts at:      0     217     549 
   Size:        217     332
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     436     549 
   Size:        436     113
 
Tth111I GACn'n_nGTC

Cuts at:      0       9     549 
   Size:          9     540
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     441     549 
   Size:        441     108
 

 Enzymes that do cut:

     AarI     AcuI    BanII     BmrI     BpmI    BsaAI    BseRI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
   BstEII    BstXI    BtgZI     DrdI     EaeI     EciI  EcoICRI  Eco57MI
    EcoRV     FspI    HaeII     MscI     MslI     NspI     PasI     PmlI
     PstI     SacI     SfcI    TaqII  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiWI    BsmBI
    BspEI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstYI
  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII
     EagI     EarI    EcoNI EcoO109I    EcoRI     FalI     FseI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
     TstI     XbaI     XhoI     XmaI     XmnI     ZraI