(Linear) (Six-base) MAPSORT of: KIBB6893.seq  Check: 570  from: 1  to: 552



KIBB6893.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:      0     413     552 
   Size:        413     139
 
AflIII A'CryG_T

Cuts at:      0     106     552 
   Size:        106     446
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     517     552 
   Size:        517      35
 
AvaI C'yCGr_G

Cuts at:      0     355     552 
   Size:        355     197
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     392     552 
   Size:        392     160
 
Bpu10I CC'TnA_GC

Cuts at:      0     229     552 
   Size:        229     323
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     289     552 
   Size:        289     263
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      86      89     552 
   Size:         86       3     463

  Fragments arranged by size: 

                 463      86       3
 
BseYI C'CCAG_C

Cuts at:      0      13     438     552 
   Size:         13     425     114

  Fragments arranged by size: 

                 425     114      13
 
BsiHKAI G_wGCw'C

Cuts at:      0      25     552 
   Size:         25     527
 
BsmI GAATG_Cn'

Cuts at:      0     162     379     552 
   Size:        162     217     173

  Fragments arranged by size: 

                 217     173     162
 
Bsp1286I G_dGCh'C

Cuts at:      0      25     552 
   Size:         25     527
 
BsrDI GCAATG_nn'

Cuts at:      0     423     552 
   Size:        423     129
 
BtsI GCAGTG_nn'

Cuts at:      0      25     552 
   Size:         25     527
 
DraI TTT'AAA

Cuts at:      0     267     552 
   Size:        267     285
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     269     301     552 
   Size:        269      32     251

  Fragments arranged by size: 

                 269     251      32
 
HincII GTy'rAC

Cuts at:      0      73     414     552 
   Size:         73     341     138

  Fragments arranged by size: 

                 341     138      73
 
MspA1I CmG'CkG

Cuts at:      0     508     552 
   Size:        508      44
 
NspI r_CATG'y

Cuts at:      0      58     110     552 
   Size:         58      52     442

  Fragments arranged by size: 

                 442      58      52
 
PciI A'CATG_T

Cuts at:      0     106     552 
   Size:        106     446
 
PspXI vC'TCGA_Gb

Cuts at:      0     355     552 
   Size:        355     197
 
PvuII CAG'CTG

Cuts at:      0     508     552 
   Size:        508      44
 
SalI G'TCGA_C

Cuts at:      0     412     552 
   Size:        412     140
 
SmlI C'TyrA_G

Cuts at:      0     355     552 
   Size:        355     197
 
SphI G_CATG'C

Cuts at:      0      58     552 
   Size:         58     494
 
SspI AAT'ATT

Cuts at:      0      37     552 
   Size:         37     515
 
StuI AGG'CCT

Cuts at:      0     353     552 
   Size:        353     199
 
TatI w'GTAC_w

Cuts at:      0     359     552 
   Size:        359     193
 
XhoI C'TCGA_G

Cuts at:      0     355     552 
   Size:        355     197
 
XmnI GAAnn'nnTTC

Cuts at:      0     338     552 
   Size:        338     214
 

 Enzymes that do cut:

     AccI   AflIII     AlfI     AvaI     BbsI   Bpu10I     BsaI    BseRI
    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI     BtsI     DraI    Hin4I
   HincII   MspA1I     NspI     PciI    PspXI    PvuII     SalI     SmlI
     SphI     SspI     StuI     TatI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PsrI     PstI     PvuI    RsrII     SacI    SacII    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI